PAK phosphorylates p21 RAF1 on S338

Stable Identifier
R-HSA-5624492
Type
Reaction [transition]
Species
Homo sapiens
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RAF regulation is highly complex and is not solely mediated by conformational changes but also requires phosphorylation. Several phosphorylation sites on RAF1 have been identified that are involved in its kinase activity. Two of these phosphorylation sites are serine 338 (S338) and tyrosine 340 and 341 (Y340/341). Gringhuis et al. demonstrated that ManLAM activation of CD209 (DC-SIGN) results in induced phosphorylation of RAF1 on S338 and Y340/341. The phosphorylation of S338 is mediated by p21-activated kinases (PAK). Inhibition of Rho GTPases with toxin B blocks CD209 induced phosphorylation of RAF1on S338 (Gringhuis et al. 2007, Wellbrock et al. 2004).

Literature References
PubMed ID Title Journal Year
20363321 C-type lectin DC-SIGN: an adhesion, signalling and antigen-uptake molecule that guides dendritic cells in immunity

Svajger, U, Anderluh, M, Jeras, M, Obermajer, N

Cell. Signal. 2010
15520807 The RAF proteins take centre stage

Wellbrock, C, Karasarides, M, Marais, R

Nat Rev Mol Cell Biol 2004
17462920 C-type lectin DC-SIGN modulates Toll-like receptor signaling via Raf-1 kinase-dependent acetylation of transcription factor NF-kappaB

Gringhuis, SI, den Dunnen, J, Litjens, M, van Het Hof, B, van Kooyk, Y, Geijtenbeek, TB

Immunity 2007
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Catalyst Activity
Title
protein serine/threonine kinase activity of PAK1,2,3 [cytosol]
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Orthologous Events
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