Reactome | PAK phosphorylates p21 RAF1 on S338

PAK phosphorylates p21 RAF1 on S338

Stable Identifier

R-HSA-5624492

Type

Reaction [transition]

Species

Homo sapiens

Compartment

plasma membrane, cytosol

ReviewStatus

5/5

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RAF regulation is highly complex and is not solely mediated by conformational changes but also requires phosphorylation. Several phosphorylation sites on RAF1 have been identified that are involved in its kinase activity. Two of these phosphorylation sites are serine 338 (S338) and tyrosine 340 and 341 (Y340/341). Gringhuis et al. demonstrated that ManLAM activation of CD209 (DC-SIGN) results in induced phosphorylation of RAF1 on S338 and Y340/341. The phosphorylation of S338 is mediated by p21-activated kinases (PAK). Inhibition of Rho GTPases with toxin B blocks CD209 induced phosphorylation of RAF1on S338 (Gringhuis et al. 2007, Wellbrock et al. 2004).

Literature References

PubMed ID	Title	Journal	Year
15520807	The RAF proteins take centre stage Wellbrock, C, Karasarides, M, Marais, R	Nat Rev Mol Cell Biol	2004
20363321	C-type lectin DC-SIGN: an adhesion, signalling and antigen-uptake molecule that guides dendritic cells in immunity Svajger, U, Anderluh, M, Jeras, M, Obermajer, N	Cell. Signal.	2010
17462920	C-type lectin DC-SIGN modulates Toll-like receptor signaling via Raf-1 kinase-dependent acetylation of transcription factor NF-kappaB Gringhuis, SI, den Dunnen, J, Litjens, M, van Het Hof, B, van Kooyk, Y, Geijtenbeek, TB	Immunity	2007