PDPK1 phosphorylates PKN1,2,3

Stable Identifier
R-HSA-5623667
Type
Reaction [transition]
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
General
SVG |   | PPTX  | SBGN
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

PDPK1 (PDK1) phosphorylates PKN1, PKN2 and likely PKN3 on a highly conserved threonine residue (T774 of PKN1, T816 of PKN2, T718 of PKN3) in the kinase activation loop. Although phosphorylation of PKN1, PKN2 and PKN3 at other sites may be needed for them to achieve the full catalytic activity, PDPK1-mediated phosphorylation of the activation loop is a necessary step in PKN1, PKN2 and likely PKN3 activation. This reaction happens while the PKN protein is complexed with a RHO GTPase and PIP3-bound PDPK1 (Flynn et al. 2000, Torbett et al. 2003, Dettori et al. 2009).

Literature References
PubMed ID Title Journal Year
10753910 Rho GTPase control of protein kinase C-related protein kinase activation by 3-phosphoinositide-dependent protein kinase

Flynn, P, Mellor, H, Casamassima, A, Parker, PJ

J. Biol. Chem. 2000
12783890 Hyperosmotic-induced protein kinase N 1 activation in a vesicular compartment is dependent upon Rac1 and 3-phosphoinositide-dependent kinase 1

Torbett, NE, Casamassima, A, Parker, PJ

J. Biol. Chem. 2003
19723632 Regulation of the interaction between protein kinase C-related protein kinase 2 (PRK2) and its upstream kinase, 3-phosphoinositide-dependent protein kinase 1 (PDK1)

Dettori, R, Sonzogni, S, Meyer, L, Lopez-Garcia, LA, Morrice, NA, Zeuzem, S, Engel, M, Piiper, A, Neimanis, S, Frödin, M, Biondi, RM

J. Biol. Chem. 2009
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
protein kinase C activity of RHOA,RHOB,RHOC,RAC1:GTP:PKN1-3:PDPK1:PIP3 [plasma membrane]
Physical Entity
Activity
Orthologous Events
Authored
Created