Reactome | ATAT acetylates microtubules

ATAT acetylates microtubules

Stable Identifier

R-HSA-5618328

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

4/5

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Cilia are enriched in acetylated tubulin, a marker that is associated with stability, and the kinesin motor preferentially travels on acetylated microtubules (Johnson et al, 1998; Reed et al, 2006; Cai et al, 2009). Alpha tubulin acetyltransferase (ATAT), also known as MEC17, has been shown to catalyze the acetylation of alpha tubulin at K40 (Akella et al, 2010; Shida et al, 2010; Montagnac et al, 2013; Taschner et al, 2012). ATAT preferentially acetylates polymerized alpha tubulin and may access the luminal K40 residue through 'breathing' of the microtubules protofilaments (Shida et al, 2010). ATAT was identified as an interacting protein with components of the BBSome, and siRNA knockdown of ATAT delays assembly of the primary cilium (Jin et al, 2010; Shida et al, 2010). BBIP1 is another component of the BBSome that has been shown to affect tubulin acetylation and stability, potentially through its interaction with HDAC6. BBIP1 exerts its effect on microtubule acetylation independently of its role as a component of the BBSome; it may exert its indirect effect by promoting ATAT-mediated acceleration, by counteracting HDAC6-mediated deacetylation, or by another mechanism (Loktev et al, 2008)

Literature References

PubMed ID	Title	Journal	Year
20603001	The conserved Bardet-Biedl syndrome proteins assemble a coat that traffics membrane proteins to cilia Aguiar, M, Shida, T, Jin, H, Gygi, SP, Nachury, MV, White, SR, Schulz, S, Bazan, JF	Cell	2010
9427680	The axonemal microtubules of the Chlamydomonas flagellum differ in tubulin isoform content Johnson, KA	J. Cell. Sci.	1998
23071318	Atomic resolution structure of human α-tubulin acetyltransferase bound to acetyl-CoA Vetter, M, Taschner, M, Lorentzen, E	Proc Natl Acad Sci U S A	2012
24906155	Molecular basis for age-dependent microtubule acetylation by tubulin acetyltransferase Valenstein, ML, Szyk, A, Roll-Mecak, A, Deaconescu, AM, Kormendi, V, Goodman, B, Grigorieff, N, Ziolkowska, NE, Spector, J	Cell	2014
21068373	The major alpha-tubulin K40 acetyltransferase alphaTAT1 promotes rapid ciliogenesis and efficient mechanosensation Goodman, MB, Shida, T, Cueva, JG, Nachury, MV, Xu, Z	Proc. Natl. Acad. Sci. U.S.A.	2010
20829795	MEC-17 is an alpha-tubulin acetyltransferase Gaertig, J, Starostina, NG, Lyons-Abbott, S, Morrissette, NS, Akella, JS, Kipreos, ET, Dougan, ST, Kim, J, Wloga, D	Nature	2010
24097348	?TAT1 catalyses microtubule acetylation at clathrin-coated pits Chavrier, P, Montagnac, G, Shida, T, Irondelle, M, Benmerah, A, Nachury, MV, Castro-Castro, A, Olivo-Marin, JC, Franco, M, Meas-Yedid, V	Nature	2013
19823565	Single molecule imaging reveals differences in microtubule track selection between Kinesin motors Verhey, KJ, McEwen, DP, Martens, JR, Cai, D, Meyhofer, E	PLoS Biol.	2009
17084703	Microtubule acetylation promotes kinesin-1 binding and transport Gaertig, J, Verhey, KJ, Reed, NA, Jih, GT, Cai, D, Meyhofer, E, Blasius, TL	Curr. Biol.	2006
19081074	A BBSome subunit links ciliogenesis, microtubule stability, and acetylation Scheetz, TE, Searby, CC, Loktev, AV, Zhang, Q, Sheffield, VC, Nachury, MV, Slusarski, DC, Jackson, PK, Beck, JS, Bazan, JF	Dev. Cell	2008