Assembly of IFT B complex

Stable Identifier
R-HSA-5617820
Type
Reaction [binding]
Species
Homo sapiens
Compartment
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Based on studies done in C. reinhardtii, C. elegans and mouse, the human IFT B complex likely consists of, minimally, IFT20, RABL5/IFT22, HSPB11/IFT25, IFT27, IFT46, IFT52, TRAF3IP1/IFT54, IFT74, IFT80, IFT81, IFT88, CLUAP/QILIN, IFT70/TTC30, and TTC26/IFT56, with IFT172 being an additional candidate (Follit et al, 2009; Piperno and Mead, 1997; Cole et al, 1998; Cole, 2003; Ou, 2007; Hallbritter et al, 2013; reviewed in Taschner et al, 2012). Work in C. reinhardtii and mouse suggests that IFT B consists of a salt stable core complex of IFT88, IFT81, IFT74, IFT70, IFT52, IFT46 IFT 27, IFT25 and IFT22 with peripheral, weakly associated subunits IFT 172, IFT80, IFT57, CLUAP, TTC26 and IFT20 (Lucker et al, 2005; Lucker et al, 2010; Follit et al, 2009; Bhogaraju et al, 2011). In Chlamydomonas, core components IFT81 and IFT74 have been shown to interact directly and a stable sub-complex of IFT81/74/27/25 has been demonstrated (Lucker et al, 2005; Taschner et al, 2011). Human IFT81 and IFT74 have likewise been shown to directly interact and to form a tubulin-binding complex (Bhogaraju et al, 2013). A recent study has elucidated more detail of the protein-protein interactions that direct the assembly of the IFTB complex (Taschner et al, 2014).
This reaction shows putative human IFT B proteins assembling in a single step; details of how and when this assembly occurs are not shown, nor are the specific protein-protein interactions within the complex or details of how IFT B is regulated. Moreover, this reaction shows the formation of a presumptive IFT B* complex, lacking IFT20, to allow the recruitment of IFT20 from the Golgi compartment to be depicted.

Literature References
PubMed ID Title Journal Year
19253336 Characterization of mouse IFT complex B

Follit, JA, Xu, F, Keady, BT, Pazour, GJ

Cell Motil. Cytoskeleton 2009
24140113 Defects in the IFT-B component IFT172 cause Jeune and Mainzer-Saldino syndromes in humans

Halbritter, J, Bizet, AA, Schmidts, M, Porath, JD, Braun, DA, Gee, HY, McInerney-Leo, AM, Krug, P, Filhol, E, Davis, EE, Airik, R, Czarnecki, PG, Lehman, AM, Trnka, P, Nitschké, P, Bole-Feysot, C, Schueler, M, Knebelmann, B, Burtey, S, Szabó, AJ, Tory, K, Leo, PJ, Gardiner, B, McKenzie, FA, Zankl, A, Brown, MA, Hartley, JL, Maher, ER, Li, C, Leroux, MR, Scambler, PJ, Zhan, SH, Jones, SJ, Kayserili, H, Tuysuz, B, Moorani, KN, Constantinescu, A, Krantz, ID, Kaplan, BS, Shah, JV

Am. J. Hum. Genet. 2013
25349261 Crystal structures of IFT70/52 and IFT52/46 provide insight into intraflagellar transport B core complex assembly

Taschner, M, Kotsis, F, Braeuer, P, Kuehn, EW, Lorentzen, E

J. Cell Biol. 2014
23990561 Molecular basis of tubulin transport within the cilium by IFT74 and IFT81

Bhogaraju, S, Cajanek, L, Fort, C, Blisnick, T, Weber, K, Taschner, M, Mizuno, N, Lamla, S, Bastin, P, Nigg, EA, Lorentzen, E

Science 2013
20435895 Direct interactions of intraflagellar transport complex B proteins IFT88, IFT52, and IFT46

Lucker, BF, Miller, MS, Dziedzic, SA, Blackmarr, PT, Cole, DG

J. Biol. Chem. 2010
9114011 Transport of a novel complex in the cytoplasmic matrix of Chlamydomonas flagella

Piperno, G, Mead, K

Proc. Natl. Acad. Sci. U.S.A. 1997
12795688 The intraflagellar transport machinery of Chlamydomonas reinhardtii

Cole, DG

Traffic 2003
17314406 Sensory ciliogenesis in Caenorhabditis elegans: assignment of IFT components into distinct modules based on transport and phenotypic profiles

Ou, G, Koga, M, Blacque, OE, Murayama, T, Ohshima, Y, Schafer, JC, Li, C, Yoder, BK, Leroux, MR, Scholey, JM

Mol. Biol. Cell 2007
9585417 Chlamydomonas kinesin-II-dependent intraflagellar transport (IFT): IFT particles contain proteins required for ciliary assembly in Caenorhabditis elegans sensory neurons

Cole, DG, Diener, DR, Himelblau, AL, Beech, PL, Fuster, JC, Rosenbaum, JL

J. Cell Biol. 1998
15955805 Characterization of the intraflagellar transport complex B core: direct interaction of the IFT81 and IFT74/72 subunits

Lucker, BF, Behal, RH, Qin, H, Siron, LC, Taggart, WD, Rosenbaum, JL, Cole, DG

J. Biol. Chem. 2005
22118932 Architecture and function of IFT complex proteins in ciliogenesis

Taschner, M, Bhogaraju, S, Lorentzen, E

Differentiation 2012
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