TNRC6A (GW182) possesses both a nuclear localization signal (NLS) and a nuclear export signal (NES) that enable it to shuttle between the cytoplasm and the nucleus (Nishi et al. 2013). Thus the TNRC6A:AGO2:miRNA complex is transported into the nucleus by an unknown importation mechanism (Nishi et al. 2013). (TNRC6A is exported by Exportin 1.) The interaction between AGO2 and TNRC6A affects gene silencing activity in the nucleus (Nishi et al. 2013).