AGO1,2:small RNA complexes interact with chromatin

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Reaction [binding]
Homo sapiens
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Complexes containing small RNAs and AGO1 or AGO2 are observed within the nucleus and at the inner nuclear envelope, respectively, associated with the actin cytoskeleton (Ahlenstiel et al. 2012, Huang et al. 2013). Argonaute:miRNA complexes associate with genomic regions possessing sequences that match the miRNA, possibly via RNA transcripts tethered to chromatin (Li et al. 2006, Weinber et al. 2006, Kim et al. 2008, Younger and Corey 2011). AGO2:miRNA appears to be in complexes containing DICER and TNRC6A (Gagnon et al. 2014) and AGO1 has been shown to associate with RNA polymerase II, TARBP2, and EZH2 at transcriptionally silenced promoters (Kim et al. 2006, Huang et al. 2013). AGO1 also associates with RNA polymerase II at active promoters (Huang et al. 2013). Other AGO:miRNA complexes may form similar complexes.
Association of AGO:miRNA complexes with genes may cause transcriptional activation (Li et al. 2006), transcriptional repression (Kim et al. 2008, Younger and Corey 2011), alternative splicing (Ameyar-Zazoua et al. 2012), or DNA repair (Francia et al. 2012, Wei et al. 2012). The determinants for transcriptional activation and repression are not known. Transcriptional effects are mediated through changes in histone methylation, especially methylation of histone H3 at lysine-4, lysine-9, and lysine-27 (Li et al. 2006, Kim et al. 2006, Kim et al. 2008, Younger and Corey 2011).
Literature References
PubMed ID Title Journal Year
21427083 Transcriptional gene silencing in mammalian cells by miRNA mimics that target gene promoters

Corey, DR, Younger, ST

Nucleic Acids Res. 2011
21589992 Transcriptional regulation by miRNA mimics that target sequences downstream of gene termini

Corey, DR, Younger, ST

Mol Biosyst 2011
22064859 Direct evidence of nuclear Argonaute distribution during transcriptional silencing links the actin cytoskeleton to nuclear RNAi machinery in human cells

Ahlenstiel, CL, Lim, HG, Suzuki, K, Kelleher, AD, Cooper, DA, Ishida, T

Nucleic Acids Res. 2012
24388755 RNAi factors are present and active in human cell nuclei

Chu, Y, Janowski, BA, Gagnon, KT, Li, L, Corey, DR

Cell Rep 2014
24086155 Ago1 Interacts with RNA polymerase II and binds to the promoters of actively transcribed genes in human cancer cells

Huang, V, Yu, J, Place, RF, Li, H, Li, LC, Wang, J, Qi, Z, Zheng, J

PLoS Genet. 2013
17085592 Small dsRNAs induce transcriptional activation in human cells

Enokida, H, Urakami, S, Okino, ST, Pookot, D, Place, RF, Zhao, H, Li, LC, Dahiya, R

Proc. Natl. Acad. Sci. U.S.A. 2006
22445173 A role for small RNAs in DNA double-strand break repair

Wu, Y, Ba, Z, Gao, M, White, CI, Yang, YG, Ma, Y, Rendtlew Danielsen, JM, Amiard, S, Qi, Y, Wei, W

Cell 2012
16373483 The antisense strand of small interfering RNAs directs histone methylation and transcriptional gene silencing in human cells

Ehsani, A, Morris, KV, Rossi, JJ, Weinberg, MS, Aagaard, L, Villeneuve, LM, Chen, ZX, Amarzguioui, M, Riggs, AD

RNA 2006
22722852 Site-specific DICER and DROSHA RNA products control the DNA-damage response

Francia, S, Anelli, V, Mione, M, Carninci, P, Tang, D, de Hoon, M, Saxena, A, d'Adda di Fagagna, F, Michelini, F

Nature 2012
22961379 Argonaute proteins couple chromatin silencing to alternative splicing

Mathieu, J, Fritsch, L, Ait-Si-Ali, S, Fenouil, R, Young, R, Muchardt, C, Robin, P, Rachez, C, Harel-Bellan, A, Morozova, N, Ameyar-Zazoua, M, Batsché, E, Descostes, N, Andrau, JC, Souidi, M, Hamiche, A

Nat. Struct. Mol. Biol. 2012
18852463 MicroRNA-directed transcriptional gene silencing in mammalian cells

Saetrom, P, Rossi, JJ, Snøve, O, Kim, DH

Proc. Natl. Acad. Sci. U.S.A. 2008
25313066 Argonaute-1 binds transcriptional enhancers and controls constitutive and alternative splicing in human cells

Petrillo, E, Dujardin, G, Lührmann, R, Pelisch, F, Singh, B, Valcárcel, J, Miñana, B, Blaustein, M, Agafonov, DE, Kornblihtt, AR, Buggiano, V, Srebrow, A, Bessonov, S, Gómez Acuña, L, Eyras, E, Bellora, N, Agirre, E, Bechara, E, Pozzi, B, Bertucci, P, Alló, M

Proc. Natl. Acad. Sci. U.S.A. 2014
16936726 Argonaute-1 directs siRNA-mediated transcriptional gene silencing in human cells

Rossi, JJ, Kim, DH, Villeneuve, LM, Morris, KV

Nat Struct Mol Biol 2006
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