Reactome | AGO1,2:small RNA complexes interact with chromatin

AGO1,2:small RNA complexes interact with chromatin

Stable Identifier

R-HSA-5578742

Type

Reaction [binding]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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AGO1,2:small RNA complexes interact with chromatin

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Complexes containing small RNAs and AGO1 or AGO2 are observed within the nucleus and at the inner nuclear envelope, respectively, associated with the actin cytoskeleton (Ahlenstiel et al. 2012, Huang et al. 2013). Argonaute:miRNA complexes associate with genomic regions possessing sequences that match the miRNA, possibly via RNA transcripts tethered to chromatin (Li et al. 2006, Weinber et al. 2006, Kim et al. 2008, Younger and Corey 2011). AGO2:miRNA appears to be in complexes containing DICER and TNRC6A (Gagnon et al. 2014) and AGO1 has been shown to associate with RNA polymerase II, TARBP2, and EZH2 at transcriptionally silenced promoters (Kim et al. 2006, Huang et al. 2013). AGO1 also associates with RNA polymerase II at active promoters (Huang et al. 2013). Other AGO:miRNA complexes may form similar complexes.
Association of AGO:miRNA complexes with genes may cause transcriptional activation (Li et al. 2006), transcriptional repression (Kim et al. 2008, Younger and Corey 2011), alternative splicing (Ameyar-Zazoua et al. 2012), or DNA repair (Francia et al. 2012, Wei et al. 2012). The determinants for transcriptional activation and repression are not known. Transcriptional effects are mediated through changes in histone methylation, especially methylation of histone H3 at lysine-4, lysine-9, and lysine-27 (Li et al. 2006, Kim et al. 2006, Kim et al. 2008, Younger and Corey 2011).

Literature References

PubMed ID	Title	Journal	Year
21427083	Transcriptional gene silencing in mammalian cells by miRNA mimics that target gene promoters Younger, ST, Corey, DR	Nucleic Acids Res.	2011
21589992	Transcriptional regulation by miRNA mimics that target sequences downstream of gene termini Younger, ST, Corey, DR	Mol Biosyst	2011
22064859	Direct evidence of nuclear Argonaute distribution during transcriptional silencing links the actin cytoskeleton to nuclear RNAi machinery in human cells Ahlenstiel, CL, Lim, HG, Cooper, DA, Ishida, T, Kelleher, AD, Suzuki, K	Nucleic Acids Res.	2012
24388755	RNAi factors are present and active in human cell nuclei Gagnon, KT, Li, L, Chu, Y, Janowski, BA, Corey, DR	Cell Rep	2014
24086155	Ago1 Interacts with RNA polymerase II and binds to the promoters of actively transcribed genes in human cancer cells Huang, V, Zheng, J, Qi, Z, Wang, J, Place, RF, Yu, J, Li, H, Li, LC	PLoS Genet.	2013
17085592	Small dsRNAs induce transcriptional activation in human cells Li, LC, Okino, ST, Zhao, H, Pookot, D, Place, RF, Urakami, S, Enokida, H, Dahiya, R	Proc. Natl. Acad. Sci. U.S.A.	2006
22445173	A role for small RNAs in DNA double-strand break repair Wei, W, Ba, Z, Gao, M, Wu, Y, Ma, Y, Amiard, S, White, CI, Rendtlew Danielsen, JM, Yang, YG, Qi, Y	Cell	2012
16373483	The antisense strand of small interfering RNAs directs histone methylation and transcriptional gene silencing in human cells Weinberg, MS, Villeneuve, LM, Ehsani, A, Amarzguioui, M, Aagaard, L, Chen, ZX, Riggs, AD, Rossi, JJ, Morris, KV	RNA	2006
22722852	Site-specific DICER and DROSHA RNA products control the DNA-damage response Francia, S, Michelini, F, Saxena, A, Tang, D, de Hoon, M, Anelli, V, Mione, M, Carninci, P, d'Adda di Fagagna, F	Nature	2012
22961379	Argonaute proteins couple chromatin silencing to alternative splicing Ameyar-Zazoua, M, Rachez, C, Souidi, M, Robin, P, Fritsch, L, Young, R, Morozova, N, Fenouil, R, Descostes, N, Andrau, JC, Mathieu, J, Hamiche, A, Ait-Si-Ali, S, Muchardt, C, Batsché, E, Harel-Bellan, A	Nat. Struct. Mol. Biol.	2012
18852463	MicroRNA-directed transcriptional gene silencing in mammalian cells Kim, DH, Saetrom, P, Snøve, O, Rossi, JJ	Proc. Natl. Acad. Sci. U.S.A.	2008
25313066	Argonaute-1 binds transcriptional enhancers and controls constitutive and alternative splicing in human cells Alló, M, Agirre, E, Bessonov, S, Bertucci, P, Gómez Acuña, L, Buggiano, V, Bellora, N, Singh, B, Petrillo, E, Blaustein, M, Miñana, B, Dujardin, G, Pozzi, B, Pelisch, F, Bechara, E, Agafonov, DE, Srebrow, A, Lührmann, R, Valcárcel, J, Eyras, E, Kornblihtt, AR	Proc. Natl. Acad. Sci. U.S.A.	2014
16936726	Argonaute-1 directs siRNA-mediated transcriptional gene silencing in human cells Kim, DH, Villeneuve, LM, Morris, KV, Rossi, JJ	Nat Struct Mol Biol	2006