ACSL3,4 ligate CoA to AA to form AA-CoA

Stable Identifier
Reaction [transition]
Homo sapiens
arachidonate + CoASH + ATP => arachidonoyl-CoA + AMP + pyrophosphate + H2O [ACSL4]
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Acyl-CoA synthetase long-chain family member 4 (ACSL4) associated with the endoplasmic reticulum membrane catalyses the conjugation of arachidonate (AA) with CoA to form arachidonyl-CoA (AA-CoA) (Longo et al. 2003, Meloni et al. 2003). By similarity, ACSL3 can also preferentially conjugate CoA on to AA (Yao & Ye 2008). These enzymes are involved in the activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation.

Literature References
PubMed ID Title Journal Year
18003621 Long chain acyl-CoA synthetase 3-mediated phosphatidylcholine synthesis is required for assembly of very low density lipoproteins in human hepatoma Huh7 cells

Yao, H, Ye, J

J. Biol. Chem. 2008
11889465 FACL4, encoding fatty acid-CoA ligase 4, is mutated in nonspecific X-linked mental retardation

Magi, B, des Portes, V, Renieri, A, Yntema, HG, Ropers, HH, Raynaud, M, Longo, I, Meloni, I, Moizard, MP, Chelly, J, Bruttini, M, Schaffer, JE, Bellan, C, Gomot, M, Lewis, SE, Fryns, JP, Muscettola, M, Volpi, N

Nat Genet 2002
12525535 A third MRX family (MRX68) is the result of mutation in the long chain fatty acid-CoA ligase 4 (FACL4) gene: proposal of a rapid enzymatic assay for screening mentally retarded patients

Marynen, P, Meloni, I, Schwartz, C, Ariani, F, Renieri, A, Borghgraef, M, Froyen, G, Raynaud, M, Longo, I, Pescucci, C, Fryns, JP, Frints, SG

J Med Genet 2003
Catalyst Activity

long-chain fatty acid-CoA ligase activity of ACSL3,4 [endoplasmic reticulum membrane]

Orthologous Events
Cross References
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