Reactome | MTRF1L (MTRF1a) or ICT1 binds stop codon in 55S ribosome:mRNA:peptidyl-tRNA

MTRF1L (MTRF1a) or ICT1 binds stop codon in 55S ribosome:mRNA:peptidyl-tRNA

Stable Identifier

R-HSA-5419264

Type

Reaction [binding]

Species

Homo sapiens

Compartment

mitochondrial matrix, mitochondrial inner membrane

ReviewStatus

5/5

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MTRF1L (MTRF1a) or ICT1 binds stop codon in 55S ribosome:mRNA:peptidyl-tRNA

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MTRF1L (mtRF1a) binds the stop codons UAA and UAG of the mRNA when they are in the A site of the ribosome (Soleimanpour-Lichaei 2007, Nozaki et al. 2008). (The UGA codon is recognized by the tryptophan tRNA in mitochondrial translation.) ICT1 can also bind standard stop codons in the A-site (inferred from pig mitochondrial ribosomes in Akabane et al. 2014). MTRF1 was also thought to play a role in translation termination by recognizing the unconventional termination codons AGA and AGG (Zhang and Spremulli 1998, Young et al. 2010) but frameshifting is now confirmed in the termination mechanism of these codons (Temperley et al. 2010). Structural features of MTRF1 have been reported suggesting it could recognize an empty A-site (Huynen et al. 2012) or UAA and UAG codons (Lind et al. 2013) however there is no direct experimental data to confirm these last two postulates.

Literature References

PubMed ID	Title	Journal	Year
20421313	Bioinformatic, structural, and functional analyses support release factor-like MTRF1 as a protein able to decode nonstandard stop codons beginning with adenine in vertebrate mitochondria Murphy, J, Edgar, CD, Poole, ES, Fredebohm, J, Young, DJ, Tate, WP	RNA	2010
24352605	Codon-reading specificities of mitochondrial release factors and translation termination at non-standard stop codons Aqvist, J, Lind, C, Sund, J	Nat Commun	2013
20075246	Hungry codons promote frameshifting in human mitochondrial ribosomes Lightowlers, RN, Chrzanowska-Lightowlers, ZM, Richter, R, Temperley, R, Dennerlein, S	Science	2010
18429816	HMRF1L is a human mitochondrial translation release factor involved in the decoding of the termination codons UAA and UAG Ishizawa, T, Nozaki, Y, Takeuchi, N, Matsunaga, N, Ueda, T	Genes Cells	2008
9838146	Identification and cloning of human mitochondrial translational release factor 1 and the ribosome recycling factor Zhang, Y, Spremulli, LL	Biochim. Biophys. Acta	1998
22569235	Structure based hypothesis of a mitochondrial ribosome rescue mechanism Nabuurs, SB, Duarte, I, Chrzanowska-Lightowlers, ZM, Huynen, MA	Biol. Direct	2012
17803939	mtRF1a is a human mitochondrial translation release factor decoding the major termination codons UAA and UAG Kühl, I, Passos, JF, Rorbach, J, Wydro, M, Chrzanowska-Lightowlers, ZM, Lightowlers, R, Temperley, R, Gaisne, M, Tate, W, Soleimanpour-Lichaei, HR, Bonnefoy, N	Mol. Cell	2007