MSH2:MSH3 recruits MLH1:PMS2 to mismatch and interacts with PCNA

Stable Identifier
Reaction [binding]
Homo sapiens
MutSbeta recruits MutLalpha to mismatch and interacts with PCNA
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After being activated by an insertion/deletion loop (IDL), MSH2:MSH3 (MutSbeta) recruits MLH1:PMS2 (MutLalpha) (Iyer et al. 2010, Pluciennik et al. 2013) and interacts with PCNA (Iyer et al. 2010). As inferred from yeast, more than one molecule of MLH1:PMS2 may bind per molecule of MSH2:MSH3 (Hombauer et al. 2011). MLH1:PMS2 and PCNA compete for the same binding site on MSH2:MSH3 (Iyer et al. 2010). The interaction with PCNA determines which strand of DNA will serve as template and which strand will be repaired (Zhang et al. 2005, and inferred from MSH2:MSH6).

Literature References
PubMed ID Title Journal Year
23840062 Extrahelical (CAG)/(CTG) triplet repeat elements support proliferating cell nuclear antigen loading and MutL? endonuclease activation

Burdett, V, Modrich, P, Iyer, RR, Pluciennik, A, Baitinger, C, Shi, K

Proc. Natl. Acad. Sci. U.S.A. 2013
11274057 hMSH3 and hMSH6 interact with PCNA and colocalize with it to replication foci

Jiricny, J, Marra, G, Lettieri, T, Kleczkowska, HE

Genes Dev. 2001
20154325 MutLalpha and proliferating cell nuclear antigen share binding sites on MutSbeta

Tsai, MS, Modrich, P, Beese, LS, Iyer, RR, Pluciennik, A, Genschel, J

J. Biol. Chem. 2010
22118461 Visualization of eukaryotic DNA mismatch repair reveals distinct recognition and repair intermediates

Desai, A, Hombauer, H, Kolodner, RD, Smith, CE, Campbell, CS

Cell 2011
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