Reactome | MLH1:PMS2 makes single strand incision near 1-2 base mismatch

MLH1:PMS2 makes single strand incision near 1-2 base mismatch

Stable Identifier

R-HSA-5358518

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

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MLH1:PMS2 makes single strand incision near 1-2 base mismatch

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The latent endonuclease activity of MLH1:PMS2 (MutLalpha) is activated by interaction with MSH2:MSH6 and PCNA (Kadyrov et al 2006). MLH1:PMS2 makes a nick in the replicated strand of DNA. As inferred from yeast, more than one MLH1:PMS2 may bind per MSH2:MSH6 (Hombauer et al. 2011). Strand selection of the nick is determined by interaction with PCNA, though the exact mechanism is unknown (Pluciennik et al 2010).

Literature References

PubMed ID	Title	Journal	Year
20713735	PCNA function in the activation and strand direction of MutL? endonuclease in mismatch repair Pluciennik, A, Dzantiev, L, Iyer, RR, Constantin, N, Kadyrov, FA, Modrich, P	Proc. Natl. Acad. Sci. U.S.A.	2010
16143102	Reconstitution of 5'-directed human mismatch repair in a purified system Zhang, Y, Yuan, F, Presnell, SR, Tian, K, Gao, Y, Tomkinson, AE, Gu, L, Li, GM	Cell	2005
16188885	Human mismatch repair: reconstitution of a nick-directed bidirectional reaction Constantin, N, Dzantiev, L, Kadyrov, FA, Modrich, P	J. Biol. Chem.	2005
22118461	Visualization of eukaryotic DNA mismatch repair reveals distinct recognition and repair intermediates Hombauer, H, Campbell, CS, Smith, CE, Desai, A, Kolodner, RD	Cell	2011
16873062	Endonucleolytic function of MutLalpha in human mismatch repair Kadyrov, FA, Dzantiev, L, Constantin, N, Modrich, P	Cell	2006