MLH1:PMS2 makes single strand incision near 1-2 base mismatch

Stable Identifier
R-HSA-5358518
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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The latent endonuclease activity of MLH1:PMS2 (MutLalpha) is activated by interaction with MSH2:MSH6 and PCNA (Kadyrov et al 2006). MLH1:PMS2 makes a nick in the replicated strand of DNA. As inferred from yeast, more than one MLH1:PMS2 may bind per MSH2:MSH6 (Hombauer et al. 2011). Strand selection of the nick is determined by interaction with PCNA, though the exact mechanism is unknown (Pluciennik et al 2010).

Literature References
PubMed ID Title Journal Year
20713735 PCNA function in the activation and strand direction of MutL? endonuclease in mismatch repair

Pluciennik, A, Dzantiev, L, Iyer, RR, Constantin, N, Kadyrov, FA, Modrich, P

Proc. Natl. Acad. Sci. U.S.A. 2010
16143102 Reconstitution of 5'-directed human mismatch repair in a purified system

Zhang, Y, Yuan, F, Presnell, SR, Tian, K, Gao, Y, Tomkinson, AE, Gu, L, Li, GM

Cell 2005
16188885 Human mismatch repair: reconstitution of a nick-directed bidirectional reaction

Constantin, N, Dzantiev, L, Kadyrov, FA, Modrich, P

J. Biol. Chem. 2005
22118461 Visualization of eukaryotic DNA mismatch repair reveals distinct recognition and repair intermediates

Hombauer, H, Campbell, CS, Smith, CE, Desai, A, Kolodner, RD

Cell 2011
16873062 Endonucleolytic function of MutLalpha in human mismatch repair

Kadyrov, FA, Dzantiev, L, Constantin, N, Modrich, P

Cell 2006
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
endonuclease activity of MLH1:PMS2:MSH2:MSH6:ATP:PCNA:DNA containing 1-2 base mismatch [nucleoplasm]
Physical Entity
Activity
Orthologous Events
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Reviewed
Created