Reactome | DPH5 transfers four methyl groups from AdoMet to aminocarboxypropyl EEF2

DPH5 transfers four methyl groups from AdoMet to aminocarboxypropyl EEF2

Stable Identifier

R-HSA-5358484

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

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DPH5 transfers four methyl groups from AdoMet to aminocarboxypropyl EEF2

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Cytosolic diphthamide biosynthesis protein 5 (DPH5) transfers four methyl groups from S-adenosylmethionine (AdoMet) to elongation factor 2 (EEF2) whose histidine residue at position 715 has been conjugated with a 3-amino 3-carboxypropyl group, forming methylated diphthine EEF2 and S-adenosylhomocysteine (AdoHcy). DPH5 activity has been identified in cells of diverse eukaryotic species including humans and has been characterized in detail in budding yeast (Liu et al. 2004; Matteakis et al. 1992; Moehring & Moehring 1988).

Literature References

PubMed ID	Title	Journal	Year
3346227	The post-translational trimethylation of diphthamide studied in vitro Moehring, JM, Moehring, TJ	J. Biol. Chem.	1988
1508200	DPH5, a methyltransferase gene required for diphthamide biosynthesis in Saccharomyces cerevisiae Mattheakis, LC, Shen, WH, Collier, RJ	Mol. Cell. Biol.	1992
15485916	Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2 Liu, S, Milne, GT, Kuremsky, JG, Fink, GR, Leppla, SH	Mol. Cell. Biol.	2004