DPH6 ligates ammonium to diphthine-EEF2

Stable Identifier
Reaction [transition]
Homo sapiens
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Cytosolic diphthamide biosynthesis protein 6 (DPH6) ligates an ammonium ion to diphthine-EEF2 to generate diphthamide-EEF2 in a reaction coupled to the hydrolysis of ATP to yield AMP and PPi (Su et al. 2012; Uthman et al. 2013; Wei et al. 2013).

Literature References
PubMed ID Title Journal Year
23468660 The amidation step of diphthamide biosynthesis in yeast requires DPH6, a gene identified through mining the DPH1-DPH5 interaction network

Uthman, S, Schaffrath, R, Stark, MJ, Liu, S, ten Have, S, Scheidt, V, Giorgini, F, Bär, C

PLoS Genet. 2013
23169644 Chemogenomic approach identified yeast YLR143W as diphthamide synthetase

Chen, W, Zhang, S, Su, X, Lin, Z, Lin, H, Jiang, H

Proc. Natl. Acad. Sci. U.S.A. 2012
23486472 A modified form of diphthamide causes immunotoxin resistance in a lymphoma cell line with a deletion of the WDR85 gene

Chertov, O, Wayne, AS, Pastan, I, Wei, H, Colantonio, S, Bera, TK, Xiang, L

J. Biol. Chem. 2013
Catalyst Activity

diphthine-ammonia ligase activity of DPH6 [cytosol]

Orthologous Events
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