UHRF1 binds chromatin with hemimethylated cytosine

Stable Identifier
R-HSA-5334099
Type
Reaction [binding]
Species
Homo sapiens
Compartment
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UHRF1 (also known as Np95) preferentially binds hemimethylated CG dinucleotides in DNA via its SRA domain (Avvakumov et al. 2008, Qian et al. 2008, and inferred from the mouse homolog). The UHRF1-bound unmethylated cytosine base is flipped out of the DNA helix and into a pocket of UHRF1 (Avvakumov et al. 2008). UHRF1 also binds dimethylated and trimethylated lysine-9 of histone H3 through its tandem Tudor domain (Nady et al. 2011, Rothbart et al. 2012, Rothbart et al. 2013, Cheng et al. 2013) and unmethylated histone H3 through its PHD domain (Hu et al. 2011, Wang et al. 2011, Rajakumara et al. 2011, Cheng et al. 2013).

Literature References
PubMed ID Title Journal Year
18772889 Structural basis for recognition of hemi-methylated DNA by the SRA domain of human UHRF1

Avvakumov, GV, Walker, JR, Xue, S, Li, Y, Duan, S, Bronner, C, Arrowsmith, CH, Dhe-Paganon, S

Nature 2008
21489993 Recognition of multivalent histone states associated with heterochromatin by UHRF1 protein

Nady, N, Lemak, A, Walker, JR, Avvakumov, GV, Kareta, MS, Achour, M, Xue, S, Duan, S, Allali-Hassani, A, Zuo, X, Wang, YX, Bronner, C, Chédin, F, Arrowsmith, CH, Dhe-Paganon, S

J. Biol. Chem. 2011
21808300 Crystal structure of PHD domain of UHRF1 and insights into recognition of unmodified histone H3 arginine residue 2

Hu, L, Li, Z, Wang, P, Lin, Y, Xu, Y

Cell Res. 2011
23161542 Structural insight into coordinated recognition of trimethylated histone H3 lysine 9 (H3K9me3) by the plant homeodomain (PHD) and tandem tudor domain (TTD) of UHRF1 (ubiquitin-like, containing PHD and RING finger domains, 1) protein

Cheng, J, Yang, Y, Fang, J, Xiao, J, Zhu, T, Chen, F, Wang, P, Li, Z, Yang, H, Xu, Y

J. Biol. Chem. 2013
21808299 Structural basis for site-specific reading of unmodified R2 of histone H3 tail by UHRF1 PHD finger

Wang, C, Shen, J, Yang, Z, Chen, P, Zhao, B, Hu, W, Lan, W, Tong, X, Wu, H, Li, G, Cao, C

Cell Res. 2011
17673620 UHRF1 plays a role in maintaining DNA methylation in mammalian cells

Bostick, M, Kim, JK, Estève, PO, Clark, A, Pradhan, S, Jacobsen, SE

Science 2007
21777816 PHD finger recognition of unmodified histone H3R2 links UHRF1 to regulation of euchromatic gene expression

Rajakumara, E, Wang, Z, Ma, H, Hu, L, Chen, H, Lin, Y, Guo, R, Wu, F, Li, H, Lan, F, Shi, YG, Xu, Y, Patel, DJ, Shi, Y

Mol. Cell 2011
23752590 Multivalent histone engagement by the linked tandem Tudor and PHD domains of UHRF1 is required for the epigenetic inheritance of DNA methylation

Rothbart, SB, Dickson, BM, Ong, MS, Krajewski, K, Houliston, S, Kireev, DB, Arrowsmith, CH, Strahl, BD

Genes Dev. 2013
18945682 Structure and hemimethylated CpG binding of the SRA domain from human UHRF1

Qian, C, Li, S, Jakoncic, J, Zeng, L, Walsh, MJ, Zhou, MM

J Biol Chem 2008
23022729 Association of UHRF1 with methylated H3K9 directs the maintenance of DNA methylation

Rothbart, SB, Krajewski, K, Nady, N, Tempel, W, Xue, S, Badeaux, AI, Barsyte-Lovejoy, D, Martinez, JY, Bedford, MT, Fuchs, SM, Arrowsmith, CH, Strahl, BD

Nat. Struct. Mol. Biol. 2012
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