botG LC cleaves target cell VAMP1

Stable Identifier
R-HSA-5250978
Type
Reaction [transition]
Species
Homo sapiens
Related Species
Clostridium botulinum
Compartment
synaptic vesicle membrane, cytosol
ReviewStatus
5/5
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botG LC cleaves target cell VAMP1
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Botulinum toxin type G light chain (botG LC), in the cytosol of a target cell, catalyzes the removal of an aminoterminal peptide from vesicle-associated membrane protein 1 (VAMP1). botG LC is a zinc metalloprotease (Schiavo et al. 1994; Yamasaki et al. 1994). VAMP1 is associated with the cytosolic face of the target cell synaptic vesicle and is required for vesicle docking and exocytosis.Its cleavage by botulinum toxin blocks synaptic vesicle fusion with the plasma membrane and neurotransmitter release (Sudhof et al, 1993; Sudhof 2004).
Literature References
PubMed ID Title Journal Year
7527117 Mechanism of action of tetanus and botulinum neurotoxins

Montecucco, C, Schiavo, G

Mol Microbiol 1994
7910017 Botulinum neurotoxin type G proteolyses the Ala81-Ala82 bond of rat synaptobrevin 2

Yamasaki, S, Binz, T, Hayashi, T, Szabo, E, Yamasaki, N, Eklund, M, Jahn, R, Niemann, H

Biochem. Biophys. Res. Commun. 1994
8051110 Botulinum G neurotoxin cleaves VAMP/synaptobrevin at a single Ala-Ala peptide bond

Schiavo, G, Malizio, C, Trimble, WS, Polverino de Laureto, P, Milan, G, Sugiyama, H, Johnson, EA, Montecucco, C

J. Biol. Chem. 1994
15217342 The synaptic vesicle cycle

Südhof, TC

Annu Rev Neurosci 2004
Participants
Input
Output
Participates
as an event of
Catalyst Activity

metalloendopeptidase activity of botG LC:Zn2+ [cytosol]

Physical Entity
botG LC:Zn2+ [cytosol] (Clostridium botulinum)
Activity
metalloendopeptidase activity (GO:0004222)
Disease
Name Identifier Synonyms
botulism DOID:11976 Botulism (disorder), Intoxication with Clostridium botulinum toxin, Botulism, Infection due to clostridium botulinum, Botulism, Foodborne botulism, botulism, Botulism, Botulism, Botulism, Food poisoning due to Clostridium botulinum, Botulism poisoning
Cross References
Mondo
Authored
Krupa, S  (2006-06-15)
Gopinathrao, G  (2006-06-15)
Reviewed
Ichtchenko, K  (2007-08-03)
Thirunavukkarasu, N  (2014-11-18)
Sharma, S  (2014-11-18)
Created
D'Eustachio, P  (2014-02-01)
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