Reactome | RANBP2 SUMOylates PML with SUMO1

RANBP2 SUMOylates PML with SUMO1

Stable Identifier

R-HSA-5228508

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm, nuclear envelope

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RANBP2 of the nuclear pore complex SUMOylates PML with SUMO1 at lysine-65, lysine-160, and lysine-490 (Sternsdorf et al. 1997, Kamitani et al. 1998, Duprez et al. 1999). SUMOylated PML is observed during interphase but not during mitosis (Everett et al. 1999). RANBP2 contains both a binding site for SUMO1 and a binding site for UBE2I (Tatham et al. 2005). The binding site for SUMO1 may play a role in SUMOylation of PML with SUMO1. Knockdown of RANBP2 reduces the number of PML bodies (Saitoh et al. 2006).

Literature References

PubMed ID	Title	Journal	Year
9756909	Identification of three major sentrinization sites in PML Wada, H, Kamitani, T, Yeh, ET, Fukuda-Kamitani, T, Nguyen, HP, Kito, K	J. Biol. Chem.	1998
9412458	Evidence for covalent modification of the nuclear dot-associated proteins PML and Sp100 by PIC1/SUMO-1 Sternsdorf, T, Will, H, Jensen, K	J. Cell Biol.	1997
16688858	In situ SUMOylation analysis reveals a modulatory role of RanBP2 in the nuclear rim and PML bodies Nakao, M, Tachibana, T, Uchimura, Y, Saitoh, N, Saitoh, H, Sugahara, S	Exp. Cell Res.	2006
9452416	Covalent modification of PML by the sentrin family of ubiquitin-like proteins Kamitani, T, Yeh, ET, Fukuda-Kamitani, T, Nguyen, HP, Kito, K	J. Biol. Chem.	1998
21098080	A novel proteomics approach to identify SUMOylated proteins and their modification sites in human cells Chelbi-Alix, MK, Meloche, S, Bonneil, E, Galisson, F, Courcelles, M, Thibault, P, Mahrouche, L	Mol. Cell Proteomics	2011
10574707	Cell cycle regulation of PML modification and ND10 composition Sternsdorf, T, Orr, A, van Driel, R, Everett, RD, Lomonte, P	J. Cell. Sci.	1999
15608651	Unique binding interactions among Ubc9, SUMO and RanBP2 reveal a mechanism for SUMO paralog selection Kim, S, Tatham, MH, Chen, Y, Hay, RT, Jaffray, E, Song, J	Nat. Struct. Mol. Biol.	2005
9885291	SUMO-1 modification of the acute promyelocytic leukaemia protein PML: implications for nuclear localisation Lallemand-Breitenbach, V, de Thé, H, Freemont, PS, Duprez, E, Saurin, AJ, Desterro, JM, Solomon, E, Boddy, MN, Hay, RT, Howe, K	J. Cell. Sci.	1999