RANBP2 SUMOylates PML with SUMO1

Stable Identifier
Reaction [transition]
Homo sapiens
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RANBP2 of the nuclear pore complex SUMOylates PML with SUMO1 at lysine-65, lysine-160, and lysine-490 (Sternsdorf et al. 1997, Kamitani et al. 1998, Duprez et al. 1999). SUMOylated PML is observed during interphase but not during mitosis (Everett et al. 1999). RANBP2 contains both a binding site for SUMO1 and a binding site for UBE2I (Tatham et al. 2005). The binding site for SUMO1 may play a role in SUMOylation of PML with SUMO1. Knockdown of RANBP2 reduces the number of PML bodies (Saitoh et al. 2006).
Literature References
PubMed ID Title Journal Year
9756909 Identification of three major sentrinization sites in PML

Wada, H, Kamitani, T, Yeh, ET, Fukuda-Kamitani, T, Nguyen, HP, Kito, K

J. Biol. Chem. 1998
21098080 A novel proteomics approach to identify SUMOylated proteins and their modification sites in human cells

Chelbi-Alix, MK, Meloche, S, Bonneil, E, Galisson, F, Courcelles, M, Thibault, P, Mahrouche, L

Mol. Cell Proteomics 2011
9452416 Covalent modification of PML by the sentrin family of ubiquitin-like proteins

Kamitani, T, Yeh, ET, Fukuda-Kamitani, T, Nguyen, HP, Kito, K

J. Biol. Chem. 1998
15608651 Unique binding interactions among Ubc9, SUMO and RanBP2 reveal a mechanism for SUMO paralog selection

Kim, S, Tatham, MH, Chen, Y, Hay, RT, Jaffray, E, Song, J

Nat. Struct. Mol. Biol. 2005
9412458 Evidence for covalent modification of the nuclear dot-associated proteins PML and Sp100 by PIC1/SUMO-1

Sternsdorf, T, Will, H, Jensen, K

J. Cell Biol. 1997
16688858 In situ SUMOylation analysis reveals a modulatory role of RanBP2 in the nuclear rim and PML bodies

Nakao, M, Tachibana, T, Uchimura, Y, Saitoh, N, Saitoh, H, Sugahara, S

Exp. Cell Res. 2006
9885291 SUMO-1 modification of the acute promyelocytic leukaemia protein PML: implications for nuclear localisation

Lallemand-Breitenbach, V, de Thé, H, Freemont, PS, Duprez, E, Saurin, AJ, Desterro, JM, Solomon, E, Boddy, MN, Hay, RT, Howe, K

J. Cell. Sci. 1999
10574707 Cell cycle regulation of PML modification and ND10 composition

Sternsdorf, T, Orr, A, van Driel, R, Everett, RD, Lomonte, P

J. Cell. Sci. 1999
Catalyst Activity

SUMO transferase activity of Nuclear Pore Complex (NPC) [nuclear envelope]

Orthologous Events
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