DAG and Ca+2 bind to PKC and tether it to membrane

Stable Identifier
R-HSA-5223304
Type
Reaction [binding]
Species
Homo sapiens
Compartment
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PKC contains an N-terminal C2 like domain, a pseudosubstrate (PS), DAG binding (C1) domain and a C-terminal kinase domain. The PS sequence resembles an ideal substrate with the exception that it contains an alanine residue instead of a substrate serine residue, is bound to the kinase domain in the resting state. As a result, PKC is maintained in a closed inactive state, which is inaccessible to cellular substrates (Colon-Gonzalez & Kazanietz 2006). Diacylglycerol (DAG) produced by activated lipins (LPIN1, LPIN2, LPIN3) leads to the activation of PKC (PRKCA and PRKCB) and their translocation from the nucleoplasm to the nuclear envelope where they can phosphorylate lamins (Mall et al. 2012). PKCs are tethered to the membrane through DAG binding to the C1 domain and this confers a high-affinity interaction between PKC and the membrane. This leads to a massive conformational change that releases the PS domain from the catalytic site and the system becomes both competent and accessible (Colon-Gonzalez & Kazanietz 2006).

Literature References
PubMed ID Title Journal Year
16861033 C1 domains exposed: from diacylglycerol binding to protein-protein interactions

Colón-González, F, Kazanietz, MG

Biochim. Biophys. Acta 2006
22986494 Mitotic lamin disassembly is triggered by lipid-mediated signaling

Mall, M, Walter, T, Gorjánácz, M, Davidson, IF, Nga Ly-Hartig, TB, Ellenberg, J, Mattaj, IW

J. Cell Biol. 2012
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