RANBP2 SUMOylates CDCA8 (Borealin) and PIAS3 SUMOylates AURKB (Aurora-B)

Stable Identifier
Reaction [omitted]
Homo sapiens
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During early mitosis (before metaphase) RANBP2 in the RANBP2:RANGAP-SUMO:UBC9 complex SUMOylates CDCA8 (Borealin) with SUMO2,3 at unknown lysine residues (Klein et al. 2009, Fernandez-Miranda et al. 2010, Ban et al. 2011, Werner et al. 2012, Hendriks et al. 2014). CDCA8 can also be SUMOylated with SUMO1 but SUMO2,3 is observed to predominate in vivo. At this time PIAS3 also SUMOylates AURKB (Aurora-B) at lysine-202 with SUMO2,3. The SUMOylated complex is observed in the cytosol after the nuclear envelope has broken down. As inferred from mouse, failure to SUMOylate AURKB causes defective centromeric function and abnormal chromosome segregation (Fernandez-Miranda et al. 2010).

Literature References
PubMed ID Title Journal Year
18946085 RanBP2 and SENP3 function in a mitotic SUMO2/3 conjugation-deconjugation cycle on Borealin

Nigg, EA, Haindl, M, Klein, UR, Muller, S

Mol. Biol. Cell 2009
22464730 The RanBP2/RanGAP1*SUMO1/Ubc9 complex is a multisubunit SUMO E3 ligase

Melchior, F, Flotho, A, Werner, A

Mol. Cell 2012
25218447 Uncovering global SUMOylation signaling networks in a site-specific manner

Yang, B, Hendriks, IA, Verlaan-de Vries, M, Vertegaal, AC, D'Souza, RC, Mann, M

Nat. Struct. Mol. Biol. 2014
21554500 Mitotic kinase Aurora-B is regulated by SUMO-2/3 conjugation/deconjugation during mitosis

Urano, T, Ban, R, Nishida, T

Genes Cells 2011
20663916 SUMOylation modulates the function of Aurora-B kinase

Pérez De Castro, I, Ruchaud, S, Montoya, G, Earnshaw, WC, Fernández-Miranda, G, Aguirre-Portolés, C, Fant, X, Carmena, M, Malumbres, M

J. Cell. Sci. 2010
Event Information
Go Biological Process
Catalyst Activity

SUMO transferase activity of RANBP2:RANGAP1-SUMO1:UBE2I:Nuclear Pore Complex (NPC) [nuclear envelope]

SUMO transferase activity of PIAS3 [nucleoplasm]

Orthologous Events
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