RANBP2 SUMOylates CDCA8 (Borealin) and PIAS3 SUMOylates AURKB (Aurora-B)

Stable Identifier
R-HSA-4655355
Type
Reaction [omitted]
Species
Homo sapiens
Compartment
cytosol, nucleoplasm
ReviewStatus
5/5
Locations in the PathwayBrowser
Expand all
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
RANBP2 SUMOylates CDCA8 (Borealin) and PIAS3 SUMOylates AURKB (Aurora-B)
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout
During early mitosis (before metaphase) RANBP2 in the RANBP2:RANGAP-SUMO:UBC9 complex SUMOylates CDCA8 (Borealin) with SUMO2,3 at unknown lysine residues (Klein et al. 2009, Fernandez-Miranda et al. 2010, Ban et al. 2011, Werner et al. 2012, Hendriks et al. 2014). CDCA8 can also be SUMOylated with SUMO1 but SUMO2,3 is observed to predominate in vivo. At this time PIAS3 also SUMOylates AURKB (Aurora-B) at lysine-202 with SUMO2,3. The SUMOylated complex is observed in the cytosol after the nuclear envelope has broken down. As inferred from mouse, failure to SUMOylate AURKB causes defective centromeric function and abnormal chromosome segregation (Fernandez-Miranda et al. 2010).
Literature References
PubMed ID Title Journal Year
18946085 RanBP2 and SENP3 function in a mitotic SUMO2/3 conjugation-deconjugation cycle on Borealin

Klein, UR, Haindl, M, Nigg, EA, Muller, S

Mol. Biol. Cell 2009
22464730 The RanBP2/RanGAP1*SUMO1/Ubc9 complex is a multisubunit SUMO E3 ligase

Werner, A, Flotho, A, Melchior, F

Mol. Cell 2012
25218447 Uncovering global SUMOylation signaling networks in a site-specific manner

Hendriks, IA, D'Souza, RC, Yang, B, Verlaan-de Vries, M, Mann, M, Vertegaal, AC

Nat. Struct. Mol. Biol. 2014
21554500 Mitotic kinase Aurora-B is regulated by SUMO-2/3 conjugation/deconjugation during mitosis

Ban, R, Nishida, T, Urano, T

Genes Cells 2011
20663916 SUMOylation modulates the function of Aurora-B kinase

Fernández-Miranda, G, Pérez De Castro, I, Carmena, M, Aguirre-Portolés, C, Ruchaud, S, Fant, X, Montoya, G, Earnshaw, WC, Malumbres, M

J. Cell. Sci. 2010
Participants
Input
Output
Participates
as an event of
Event Information
Go Biological Process
protein sumoylation (0016925)
Catalyst Activity

SUMO transferase activity of RANBP2:RANGAP1-SUMO1:UBE2I:Nuclear Pore Complex (NPC) [nuclear envelope]

Physical Entity
RANBP2:RANGAP1-SUMO1:UBE2I:Nuclear Pore Complex (NPC) [nuclear envelope] (Homo sapiens)
Activity
SUMO transferase activity (GO:0019789)

SUMO transferase activity of PIAS3 [nucleoplasm]

Physical Entity
PIAS3 [nucleoplasm] (Homo sapiens)
Activity
SUMO transferase activity (GO:0019789)
Orthologous Events
RANBP2 SUMOylates CDCA8 (Borealin) and PIAS3 SUMOylates AURKB (Aurora-B) (Bos taurus)
RANBP2 SUMOylates CDCA8 (Borealin) and PIAS3 SUMOylates AURKB (Aurora-B) (Drosophila melanogaster)
RANBP2 SUMOylates CDCA8 (Borealin) and PIAS3 SUMOylates AURKB (Aurora-B) (Gallus gallus)
RANBP2 SUMOylates CDCA8 (Borealin) and PIAS3 SUMOylates AURKB (Aurora-B) (Mus musculus)
RANBP2 SUMOylates CDCA8 (Borealin) and PIAS3 SUMOylates AURKB (Aurora-B) (Rattus norvegicus)
RANBP2 SUMOylates CDCA8 (Borealin) and PIAS3 SUMOylates AURKB (Aurora-B) (Sus scrofa)
RANBP2 SUMOylates CDCA8 (Borealin) and PIAS3 SUMOylates AURKB (Aurora-B) (Xenopus tropicalis)
Authored
May, B  (2013-09-28)
Reviewed
Zhang, XD  (2015-10-04)
Created
May, B  (2013-10-03)
Cite Us!