Groucho/TLE mediates repression of WNT target genes in part by recruiting a histone deacetlyase to the promoter. The weakly conserved central GP domain of Groucho/TLE has been shown to interact with the histone deacetylase RPD3/HDAC1 (Brantjes et al, 2001; Chen et al, 1999). Knockdown of rpd3 in Drosophila cells, or treatment of human or Drosophila cells with the histone deacetylase inhibitor Trichostatin A significantly decreases repression of a Groucho/TLE dependent reporter gene, and Groucho and RPD3 have been shown to co-localize to chromatin of target genes by ChIP leading to deacetylation of H3K9, H3K14, K4K5, H4K8 and H4K12 (Chen et al, 1999; Choi et al 1999; Winkler et al, 2010).
Winkler, CJ, Ponce, A, Courey, AJ
Choi, CY, Kim, YH, Kwon, HJ, Kim, Y
Brantjes, H, Roose, J, van De Wetering, M, Clevers, HC
Chen, G, Fernandez, J, Mische, S, Courey, AJ
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