PCNA is SUMOylated with SUMO1 at lysine-164, lysine-254, and other residues (Papouli et al. 2005, Pfander et al. 2005, Gali et al. 2012, Impens et al. 2014). SUMO1 is predominant in vivo. SUMOylation prevents double strand break formation and recombination if DNA replication stalls at lesions (Gali et al. 2012). This is comparable to the situation in Saccharomyces cerevisiae where sumoylated PCNA recruits the Srs2 helicase to prevent recombination during S phase (Pfander et al. 2005, Papouli et al. 2005). In the yeast PCNA homolog, SUMO at lysine-164 is located on the opposite face of PCNA from the face that interacts with DNA polymerase (Freudenthal et al. 2011).
Haracska, L, Burkovics, P, Gali, H, Fatyol, K, Szukacsov, V, Hajdu, I, Juhasz, S, Morocz, M
Washington, MT, Gakhar, L, Brogie, JE, Freudenthal, BD, Kondratick, CM
Papouli, E, Krejci, L, Chen, S, Ulrich, HD, Sung, P, Huttner, D, Davies, AA
Hoege, C, Moldovan, GL, Sacher, M, Pfander, B, Jentsch, S
Impens, F, Cossart, P, Radoshevich, L, Ribet, D
SUMO transferase activity of SUMO1:C93-UBE2I [cytoplasm]
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