Transcription of POU5F1 (OCT4)

Stable Identifier
R-HSA-452392
Type
Reaction [BlackBoxEvent]
Species
Homo sapiens
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The POU5F1 (OCT4) gene is transcribed to yield mRNA and the mRNA is translated to yield protein (Rao et al. 2004, Richards et al. 2004, Cauffman et al. 2005, Tai et al. 2005, Gerrard et al. 2005, Li et al. 2006, Adewumi et al. 2007,Assou etal. 2007). POU5F1 mRNA and protein are found in the cytoplasm of oocytes and cleavage-stage embryos (Cauffman et al. 2005). POU5F1 protein becomes nuclear during compaction, and protein and mRNA are present in inner cell mass and trophectoderm (Cauffman et al. 2005). Transcripts are also detectable in some differentiated tissues (Cauffman et al. 2005). POU5F1 is expressed in adult stem cells and cancers (Tai et al. 2005). POU5F1, SOX2, NANOG, SALL4, and SF-1(NR5A1) bind the promoter of the POU5F1 gene and enhance transcription (Matin et al. 2004, Chew et al. 2005, Boyer et al. 2005, Babaie et al. 2007, Greber et al. 2007, Wang et al. 2007, Yang et al. 2010, Chia et al. 2010). POU5F1 and SOX2 bind adjacent sites at the promoter and form a heterodimer on the DNA. SALL4 binds the promoter of the POU5F1 gene and activates transcription of POU5F1 (Yang et al. 2010). POU5F1 activates SALL4 expression thus forming a self-reinforcing loop. Activation-induced cytidine deaminase (AID) binds the methylated promoter of the POU5F1 gene, demethylates it, and enhances expression of POU5F1 (Bhutani et al. 2009). Hypoxia acts via HIF3A and EPAS1 (HIF2A) to enhance expression of POU5F1 (Forristal et al. 2010). LIN28 binds the POU5F1 mRNA and increases translation (Qiu et al. 2009).

Literature References
PubMed ID Title Journal Year
14688391 The transcriptome profile of human embryonic stem cells as defined by SAGE

Richards, M, Bongso, A, Tan, SP, Chan, WK, Tan, JH

Stem Cells 2004
16153702 Core transcriptional regulatory circuitry in human embryonic stem cells

Gifford, DK, Jaenisch, R, Lee, TI, Young, RA, Kumar, RM, Guenther, MG, Levine, SS, Boyer, LA, Jenner, RG, Cole, MF, Zucker, JP, Murray, HL, Johnstone, SE, Melton, DA

Cell 2005
18029452 Induced pluripotent stem cell lines derived from human somatic cells

Ruotti, V, Antosiewicz-Bourget, J, Thomson, JA, Vodyanik, MA, Stewart, R, Tian, S, Yu, J, Slukvin, II, Nie, J, Jonsdottir, GA, Frane, JL, Smuga-Otto, K

Science 2007
15342930 Specific knockdown of Oct4 and beta2-microglobulin expression by RNA interference in human embryonic stem cells and embryonic carcinoma cells

Andrews, PW, Matin, MM, Draper, JS, Gokhale, PJ, Moore, HD, Bahrami, AR, Morton, I, Walsh, JR

Stem Cells 2004
19755485 Hypoxia inducible factors regulate pluripotency and proliferation in human embryonic stem cells cultured at reduced oxygen tensions

Houghton, FD, Forristal, CE, Oreffo, RO, Wright, KL, Hanley, NA

Reproduction 2010
17204602 A meta-analysis of human embryonic stem cells transcriptome integrated into a web-based expression atlas

Le Carrour, T, Hamamah, S, De Vos, J, Ström, S, Hovatta, O, Assou, S, Klein, B, Marty, S, Réme, T, Hugnot, JP, Gabelle, A, Gasca, S, Nadal, L, Tondeur, S, Pantesco, V

Stem Cells 2007
17068183 Analysis of Oct4-dependent transcriptional networks regulating self-renewal and pluripotency in human embryonic stem cells

Adjaye, J, Herwig, R, Burdon, T, Brink, TC, Lehrach, H, Greber, B, Wruck, W, Groth, D, Babaie, Y

Stem Cells 2007
15513931 Oct4 expression in adult human stem cells: evidence in support of the stem cell theory of carcinogenesis

Olson, LK, Trosko, JE, Chang, CC, Webster, JD, Kiupel, M, Tai, MH

Carcinogenesis 2005
16978057 Characterization and gene expression profiling of five new human embryonic stem cell lines derived in Taiwan

Tseng, CN, Liu, YH, Li, SS, Lee, TY, Singh, S, Chung, TL

Stem Cells Dev 2006
20027182 Reprogramming towards pluripotency requires AID-dependent DNA demethylation

Brady, JJ, Bhutani, N, Blau, HM, Sacco, A, Damian, M, Corbel, SY

Nature 2009
15695770 Oct-4 mRNA and protein expression during human preimplantation development

Cauffman, G, Van Steirteghem, A, Van de Velde, H, Liebaers, I

Mol Hum Reprod 2005
19966271 Lin28-mediated post-transcriptional regulation of Oct4 expression in human embryonic stem cells

Peng, S, Huang, Y, Qiu, C, Wang, J, Ma, Y

Nucleic Acids Res 2009
15625129 Stably transfected human embryonic stem cell clones express OCT4-specific green fluorescent protein and maintain self-renewal and pluripotency

Zhao, D, Gerrard, L, Clark, AJ, Cui, W

Stem Cells 2005
15493035 Comparative transcriptional profiling of two human embryonic stem cell lines

Calhoun, JD, Stice, SL, Clark, JK, Rekaya, R, Rao, RR, Qin, X

Biotechnol Bioeng 2004
17506876 Silencing of core transcription factors in human EC cells highlights the importance of autocrine FGF signaling for self-renewal

Lehrach, H, Greber, B, Adjaye, J

BMC Dev. Biol. 2007
20953172 A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity

Chan, YS, Lufkin, T, Jiang, J, Yang, L, Moreau, D, Clarke, ND, Lu, X, Orlov, YL, Soh, BS, Huss, M, Lau, MS, Lim, B, Feng, B, Kumar, P, Chia, NY, Ng, HH, Kraus, P, Bard, F, Li, P

Nature 2010
17572666 Characterization of human embryonic stem cell lines by the International Stem Cell Initiative

Ahrlund-Richter, L, Mallon, BS, Vuoristo, S, McKay, RD, Nakatsuji, N, Nagy, A, Blum, B, Mileikovsky, M, Gertow, K, Michalska, AE, Johnson, JL, Sherrer, ES, Mikkola, M, Benvenisty, N, O'Brien, CM, Olsson, C, Vintersten, K, Mummery, CL, Emanuelsson, K, Berry, LS, Ward, D, Siemen, H, Bello, PA, van den Brink, S, Patel, M, Brooking, J, Mayshar, Y, Robins, AJ, Gokhale, PJ, Ford, A, Pera, RA, Piekarczyk, MS, Szatkiewicz, J, Jones, M, International Stem Cell, Initiative, Gertsenstein, M, Fleck, RA, Lebrin, F, Corbel, M, Suemori, H, Semb, H, Dvorak, P, Knowles, BB, Moore, HD, Amit, M, Hyllner, J, Andrews, PW, Itskovitz-Eldor, J, Pera, MF, Zhang, W, King, BL, Rossant, J, Hovatta, O, Hamilton, RS, Imreh, MP, Schulz, TC, Draper, JS, Patel, H, Minger, SL, Reubinoff, BE, Chen, KG, Otonkoski, T, Lako, M, Tuuri, T, Passier, R, Weaver, TA, Pedersen, R, Damjanov, I, Park, KY, Hampl, A, Manning, D, Stacey, GN, Adewumi, O, Rugg-Gunn, P, Jackson, J, Choo, AB, Churchill, GA, Beighton, G, Healy, LE, Turetsky, T, Oh, SK, Bevan, S, Aflatoonian, B, Kee, K, Stojkovic, M, Young, LA

Nat Biotechnol 2007
20505821 A novel SALL4/OCT4 transcriptional feedback network for pluripotency of embryonic stem cells

Chai, L, Yang, J, Gao, C, Ma, Y

PLoS One 2010
17628018 Zfp206 is a transcription factor that controls pluripotency of embryonic stem cells

Wong, KY, Robson, P, Lim, L, Teh, CH, Kueh, JL, Lufkin, T, Wang, ZX, Li, P, Stanton, LW, Rossbach, M

Stem Cells 2007
15988017 Reciprocal transcriptional regulation of Pou5f1 and Sox2 via the Oct4/Sox2 complex in embryonic stem cells

Robson, P, Tam, WL, Ang, YS, Lim, B, Loh, YH, Ng, HH, Chen, X, Li, P, Zhang, W, Yeap, LS, Chew, JL

Mol Cell Biol 2005
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