Activated NLK phosphorylates TCF7L2 and LEF1 at serine and threonine residues that lie in the central region between the DNA-binding and beta-catenin interaction domains. Phosphorylation does not affect the ability of TCF/LEF to bind to beta-catenin, but the phosphorylated complex is not able to bind to target DNA or activate transcription as assessed by EMSA and reporter gene assays (Ishitani et al, 2003b).
Ishitani, T, Ninomiya-Tsuji, J, Matsumoto, K
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