CERT1-2 complex binds ceramide

Stable Identifier
Reaction [binding]
Homo sapiens
CERT protein binds ceramide associated with the endoplasmic reticulum membrane
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CERT1-2 (ceramide transfer protein, isoform 2), an isoform of COL4A3BP, mediates the translocation of ceramides from the endoplasmic reticulum (ER) membrane to the membrane of the Golgi apparatus at the ER-Golgi membrane contact sites (MCS). Immunoprecipitation experiments suggest that CERT1-2 is associated with the ER membrane as part of a complex with PPM1L (protein phosphatase 1-like) (Saito et al. 2008) and VAPA or VAPB (VAMP-associated proteins A or B) (Kawano et al. 2006). The carboxyterminal START domain of CERT1-2 protein specifically binds ceramides (Hanada et al. 2003; Kudo et al. 2008). Non-vesicular transport of ceramide from endoplasmic reticulum to Golgi membranes is essential for cellular lipid homeostasis (reviewed by Olaiyoye et al., 2012; Kumagai & Hanada, 2019). While there is no direct support for the transfer of other ceramides than Cer(d18:1/16:0), it makes sense to assume other ceramides are transported, as well.

Literature References
PubMed ID Title Journal Year
18165232 Protein phosphatase 2Cepsilon is an endoplasmic reticulum integral membrane protein that dephosphorylates the ceramide transport protein CERT to enhance its association with organelle membranes

Tamura, S, Saito, S, Kobayashi, T, Echigo, S, Kawano, M, Matsui, H, Hanada, K, Tomishige, N, Kumagai, K

J Biol Chem 2008
18184806 Structural basis for specific lipid recognition by CERT responsible for nonvesicular trafficking of ceramide

Wakatsuki, S, Kato, R, Yamaji, T, Hanada, K, Tomishige, N, Kumagai, K, Nishijima, M, Kudo, N

Proc Natl Acad Sci U S A 2008
14685229 Molecular machinery for non-vesicular trafficking of ceramide

Yasuda, S, Miura, Y, Kawano, M, Fukasawa, M, Kumagai, K, Hanada, K, Nishijima, M

Nature 2003
31254361 Structure, functions and regulation of CERT, a lipid-transfer protein for the delivery of ceramide at the ER-Golgi membrane contact sites

Hanada, K, Kumagai, K

FEBS Lett 2019
22226883 Integration of non-vesicular and vesicular transport processes at the Golgi complex by the PKD-CERT network

Hausser, A, Olayioye, MA

Biochim Biophys Acta 2012
16895911 Efficient trafficking of ceramide from the endoplasmic reticulum to the Golgi apparatus requires a VAMP-associated protein-interacting FFAT motif of CERT

Kawano, M, Hanada, K, Kumagai, K, Nishijima, M

J Biol Chem 2006
This event is regulated