Reactome | CERT1-2 complex binds ceramide

CERT1-2 complex binds ceramide

Stable Identifier

R-HSA-429699

Type

Reaction [binding]

Species

Homo sapiens

Compartment

endoplasmic reticulum membrane

Synonyms

CERT protein binds ceramide associated with the endoplasmic reticulum membrane

ReviewStatus

5/5

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CERT1-2 (ceramide transfer protein, isoform 2), an isoform of COL4A3BP, mediates the translocation of ceramides from the endoplasmic reticulum (ER) membrane to the membrane of the Golgi apparatus at the ER-Golgi membrane contact sites (MCS). Immunoprecipitation experiments suggest that CERT1-2 is associated with the ER membrane as part of a complex with PPM1L (protein phosphatase 1-like) (Saito et al. 2008) and VAPA or VAPB (VAMP-associated proteins A or B) (Kawano et al. 2006). The carboxyterminal START domain of CERT1-2 protein specifically binds ceramides (Hanada et al. 2003; Kudo et al. 2008). Non-vesicular transport of ceramide from endoplasmic reticulum to Golgi membranes is essential for cellular lipid homeostasis (reviewed by Olaiyoye et al., 2012; Kumagai & Hanada, 2019). While there is no direct support for the transfer of other ceramides than Cer(d18:1/16:0), it makes sense to assume other ceramides are transported, as well.

Literature References

PubMed ID	Title	Journal	Year
18165232	Protein phosphatase 2Cepsilon is an endoplasmic reticulum integral membrane protein that dephosphorylates the ceramide transport protein CERT to enhance its association with organelle membranes Tamura, S, Saito, S, Kobayashi, T, Echigo, S, Kawano, M, Matsui, H, Hanada, K, Tomishige, N, Kumagai, K	J Biol Chem	2008
18184806	Structural basis for specific lipid recognition by CERT responsible for nonvesicular trafficking of ceramide Wakatsuki, S, Kato, R, Yamaji, T, Hanada, K, Tomishige, N, Kumagai, K, Nishijima, M, Kudo, N	Proc Natl Acad Sci U S A	2008
14685229	Molecular machinery for non-vesicular trafficking of ceramide Yasuda, S, Miura, Y, Kawano, M, Fukasawa, M, Kumagai, K, Hanada, K, Nishijima, M	Nature	2003
31254361	Structure, functions and regulation of CERT, a lipid-transfer protein for the delivery of ceramide at the ER-Golgi membrane contact sites Hanada, K, Kumagai, K	FEBS Lett	2019
22226883	Integration of non-vesicular and vesicular transport processes at the Golgi complex by the PKD-CERT network Hausser, A, Olayioye, MA	Biochim Biophys Acta	2012
16895911	Efficient trafficking of ceramide from the endoplasmic reticulum to the Golgi apparatus requires a VAMP-associated protein-interacting FFAT motif of CERT Kawano, M, Hanada, K, Kumagai, K, Nishijima, M	J Biol Chem	2006