Reactome | PRKD1,2,3 phosphorylates CERT1-2

PRKD1,2,3 phosphorylates CERT1-2

Stable Identifier

R-HSA-429698

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

Synonyms

CERT + ATP => monophospho-CERT + ADP

ReviewStatus

5/5

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Cytosolic PRKD1, 2, and 3 (protein kinase D1, D2, and D3) catalyze the phosphorylation of serine residue 132 of isoform 2 of ceramide transfer protein (CERT1-2, aka COL4A3BP-2). Protein kinase D (PRKD) is a crucial regulator of secretory transport at the trans-Golgi network (TGN). Phosphorylation of COL4A3BP-2 reduces its ceramide transfer activity. PRKDs may, therefore, act as regulators of lipid homeostasis (Fugmann et al., 2007; Kumagai et al., 2014; Shimasaki et al., 2022; reviewed by Olayioye & Hausser, 2011; Kumagai & Hanada, 2019).

Literature References

PubMed ID	Title	Journal	Year
35409383	Hyperosmotic Stress Induces Phosphorylation of CERT and Enhances Its Tethering throughout the Endoplasmic Reticulum Shimasaki, K, Kumagai, K, Sakai, S, Yamaji, T, Hanada, K	Int J Mol Sci	2022
24569996	Phosphoregulation of the ceramide transport protein CERT at serine 315 in the interaction with VAMP-associated protein (VAP) for inter-organelle trafficking of ceramide in mammalian cells Kumagai, K, Kawano-Kawada, M, Hanada, K	J Biol Chem	2014
31254361	Structure, functions and regulation of CERT, a lipid-transfer protein for the delivery of ceramide at the ER-Golgi membrane contact sites Kumagai, K, Hanada, K	FEBS Lett	2019
17591919	Regulation of secretory transport by protein kinase D-mediated phosphorylation of the ceramide transfer protein Fugmann, T, Hausser, A, Schoffler, P, Schmid, S, Pfizenmaier, K, Olayioye, MA	J Cell Biol	2007
22226883	Integration of non-vesicular and vesicular transport processes at the Golgi complex by the PKD-CERT network Olayioye, MA, Hausser, A	Biochim Biophys Acta	2012