Reactome | Nonendonucleolytic RISC binds exactly matching target RNAs

Nonendonucleolytic RISC binds exactly matching target RNAs

Stable Identifier

R-HSA-426522

Type

Reaction [binding]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

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Nonendonucleolytic RISC binds exactly matching target RNAs

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RISCs containing Argonaute-1 (AGO1, EIF2C1), AGO3 (EIF2C3), and AGO4 (EIF2C4) bind to target RNAs by base-pairing between the target RNA and the guide RNA of the RISC. AGO1,3,4 do not possess ribonuclease activity therefore exact matches between the guide and the target do not result in cleavage of the target. Rather, the effect of binding is inhibition of translation followed by decay of the target RNA. Argonaute proteins have been shown to interact with ribosomal proteins and with components of processing bodies (P-bodies) where RNA degradation occurs. Direct interaction between AGO and a TNRC6 protein is required for inhibition of translation and targeting to P-bodies in vivo.

Literature References

PubMed ID	Title	Journal	Year
17632058	Molecular basis for target RNA recognition and cleavage by human RISC Schroeder, R, Ameres, SL, Martinez, J	Cell	2007
16284623	A role for the P-body component GW182 in microRNA function Liu, J, Hannon, GJ, Wohlschlegel, J, Yates, JR 3rd, Rivas, FV, Parker, R	Nat Cell Biol	2005
16289642	Identification of novel argonaute-associated proteins Urlaub, H, Landthaler, M, Lührmann, R, Tuschl, T, Peters, L, Chen, PY, Meister, G	Curr Biol	2005
17128271	Evidence that microRNAs are associated with translating messenger RNAs in human cells Nilsen, TW, Fisher, J, Maroney, PA, Yu, Y	Nat Struct Mol Biol	2006