Nonendonucleolytic RISC binds exactly matching target RNAs

Stable Identifier
Reaction [binding]
Homo sapiens
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RISCs containing Argonaute-1 (AGO1, EIF2C1), AGO3 (EIF2C3), and AGO4 (EIF2C4) bind to target RNAs by base-pairing between the target RNA and the guide RNA of the RISC. AGO1,3,4 do not possess ribonuclease activity therefore exact matches between the guide and the target do not result in cleavage of the target. Rather, the effect of binding is inhibition of translation followed by decay of the target RNA. Argonaute proteins have been shown to interact with ribosomal proteins and with components of processing bodies (P-bodies) where RNA degradation occurs. Direct interaction between AGO and a TNRC6 protein is required for inhibition of translation and targeting to P-bodies in vivo.
Literature References
PubMed ID Title Journal Year
17632058 Molecular basis for target RNA recognition and cleavage by human RISC

Schroeder, R, Ameres, SL, Martinez, J

Cell 2007
16284623 A role for the P-body component GW182 in microRNA function

Liu, J, Hannon, GJ, Wohlschlegel, J, Yates, JR 3rd, Rivas, FV, Parker, R

Nat Cell Biol 2005
16289642 Identification of novel argonaute-associated proteins

Urlaub, H, Landthaler, M, L├╝hrmann, R, Tuschl, T, Peters, L, Chen, PY, Meister, G

Curr Biol 2005
17128271 Evidence that microRNAs are associated with translating messenger RNAs in human cells

Nilsen, TW, Fisher, J, Maroney, PA, Yu, Y

Nat Struct Mol Biol 2006
Orthologous Events
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