Reactome | Endonucleolytic RISC hydrolyzes target RNAs

Endonucleolytic RISC hydrolyzes target RNAs

Stable Identifier

R-HSA-426520

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

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Endonucleolytic RISC hydrolyzes target RNAs

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Human Argonaute-2 (AGO2, EIF2C2) possesses ribonucleolytic activity in its PIWI domain and cleaves target RNAs that are exactly complementary to the guide RNA at a location around 10 nucleotides from the 5' end of the match with the guide RNA. The products of cleavage have a 5' phosphate and a 3' hydroxyl group. Both complexes containing siRNAs and miRNAs are capable of cleavage. Although Argonaute proteins interact with many other proteins, the complex of AGO2 and the guide RNA are sufficient to direct cleavage of target RNAs in vitro. In vivo, cleavage requires interaction of AGO2 with a TNRC6 protein and MOV10.

Literature References

PubMed ID	Title	Journal	Year
26140367	Human Argonaute 2 Has Diverse Reaction Pathways on Target RNAs Jo, MH, Shin, S, Jung, SR, Kim, E, Song, JJ, Hohng, S	Mol. Cell	2015
18978028	Molecular characterization of human Argonaute-containing ribonucleoprotein complexes and their bound target mRNAs Landthaler, M, Gaidatzis, D, Rothballer, A, Chen, PY, Soll, SJ, Dinic, L, Ojo, T, Hafner, M, Zavolan, M, Tuschl, T	RNA	2008
15284456	Argonaute2 is the catalytic engine of mammalian RNAi Liu, J, Carmell, MA, Rivas, FV, Marsden, CG, Thomson, JM, Song, JJ, Hammond, SM, Joshua-Tor, L, Hannon, GJ	Science	2004
17632058	Molecular basis for target RNA recognition and cleavage by human RISC Ameres, SL, Martinez, J, Schroeder, R	Cell	2007
15260970	Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs Meister, G, Landthaler, M, Patkaniowska, A, Dorsett, Y, Teng, G, Tuschl, T	Mol Cell	2004
15800637	Purified Argonaute2 and an siRNA form recombinant human RISC Rivas, FV, Tolia, NH, Song, JJ, Aragon, JP, Liu, J, Hannon, GJ, Joshua-Tor, L	Nat Struct Mol Biol	2005
16289642	Identification of novel argonaute-associated proteins Meister, G, Landthaler, M, Peters, L, Chen, PY, Urlaub, H, Lührmann, R, Tuschl, T	Curr Biol	2005
12154197	A microRNA in a multiple-turnover RNAi enzyme complex Hutvagner, G, Zamore, PD	Science	2002
19470757	Functional Dissection of Human TNRC6, GW182-related, Family of proteins Baillat, D, Shiekhattar, R	Mol Cell Biol	2009