Reactome | IRE1alpha hydrolyzes Xbp1 mRNA and Xbp1 mRNA is spliced

IRE1alpha hydrolyzes Xbp1 mRNA and Xbp1 mRNA is spliced

Stable Identifier

R-HSA-425923

Type

Reaction [omitted]

Species

Homo sapiens

Compartment

cytosol, endoplasmic reticulum membrane

ReviewStatus

5/5

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IRE1alpha hydrolyzes Xbp1 mRNA and Xbp1 mRNA is spliced

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Phosphorylated IRE1-alpha homodimers with bound ADP have endoribonuclease activity in their C-terminal (cytosolic) regions. The IRE1-alpha homodimers cleave an internal 26 nucleotide segment out of the Xbp-1 mRNA. In yeast the resulting RNAs are ligated by a tRNA ligase but the corresponding human ligase has not been identified. The cleavage and ligation leads to a frameshift in the Xbp-1 mRNA which results in a longer ORF that encodes Xbp-1 (S), the active form of the Xbp-1 transcription factor

Literature References

PubMed ID	Title	Journal	Year
11779464	XBP1 mRNA is induced by ATF6 and spliced by IRE1 in response to ER stress to produce a highly active transcription factor Yoshida, H, Matsui, T, Yamamoto, A, Okada, T, Mori, K	Cell	2001
17026957	Analysis of the XBP1 splicing mechanism using endoplasmic reticulum stress-indicators Iwawaki, T, Akai, R	Biochem Biophys Res Commun	2006
18242182	RNase domains determine the functional difference between IRE1alpha and IRE1beta Imagawa, Y, Hosoda, A, Sasaka, S, Tsuru, A, Kohno, K	FEBS Lett	2008
19622636	Unconventional splicing of XBP1 mRNA occurs in the cytoplasm during the mammalian unfolded protein response Uemura, A, Oku, M, Mori, K, Yoshida, H	J Cell Sci	2009