Formation of the IGF:IGFBP3:ALS complex

Stable Identifier
Reaction [binding]
Homo sapiens
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IGFBP3 binds IGF I or IGF II via the conserved N terminus and C terminus of IGFBP 3. IGFBP3 also binds ALS via the C terminal portion of IGFBP3. The interaction is dependent on the glycosylation of ALS.
IGFBP3, which binds most IGF in the body, is enriched in follicular fluid and found in many other tissues. IGFBP3 may be cleaved by plasmin, thrombin, Prostate specific Antigen (PSA, KLK3), Matrix Metalloprotease-1 (MMP1), and Matrix Metalloprotease-2 (MMP2). IGFBP3 also binds extracellular matrix and binding lowers its affinity for IGFs. IGFBP3 stimulates the effects of IGFs.

Literature References
PubMed ID Title Journal Year
7683646 Binding of mutants of human insulin-like growth factor II to insulin-like growth factor binding proteins 1-6

Hsieh, S, Sakano, K, Rechler, MM, Fujiwara, H, Bach, LA, Perdue, JF

J Biol Chem 1993
10026136 N-Linked glycosylation and sialylation of the acid-labile subunit. Role in complex formation with insulin-like growth factor (IGF)-binding protein-3 and the IGFs

Janosi, JB, Delhanty, PJ, Baxter, RC, Firth, SM, Bond, JJ

J Biol Chem 1999
12466191 Cellular actions of the insulin-like growth factor binding proteins

Baxter, RC, Firth, SM

Endocr Rev 2002
17047378 The role of insulin-like growth factor binding proteins

Perks, C, Holly, J

Neuroendocrinology 2006
12379487 IGF-binding proteins are multifunctional and act via IGF-dependent and -independent mechanisms

Mohan, S, Baylink, DJ

J Endocrinol 2002
1370451 Structural determinants for binary and ternary complex formation between insulin-like growth factor-I (IGF-I) and IGF binding protein-3

Cascieri, MA, Bayne, ML, Baxter, RC

J Biol Chem 1992
9497324 Insulin-like growth factor (IGF)-binding protein 5 forms an alternative ternary complex with IGFs and the acid-labile subunit

Twigg, SM, Baxter, RC

J Biol Chem 1998
10681634 Insulin-like growth factor binding protein (IGFBP)-3-bound IGF-I and IGFBP-3-bound IGF-II in growth hormone deficiency

Rivarola, MA, Belgorosky, A

Horm Res 1999
15485880 Role of N- and C-terminal residues of insulin-like growth factor (IGF)-binding protein-3 in regulating IGF complex formation and receptor activation

Yan, X, Forbes, BE, Baxter, RC, Firth, SM, McNeil, KA

J Biol Chem 2004
2476804 Structure of the Mr 140,000 growth hormone-dependent insulin-like growth factor binding protein complex: determination by reconstitution and affinity-labeling

Martin, JL, Baxter, RC

Proc Natl Acad Sci U S A 1989
7543116 Serum levels of insulin-like growth factor (IGF)-binding protein-3 (IGFBP-3) in healthy infants, children, and adolescents: the relation to IGF-I, IGF-II, IGFBP-1, IGFBP-2, age, sex, body mass index, and pubertal maturation

Hall, K, Skakkebaek, NE, Müller, J, Blum, WF, Michaelsen, KF, Bang, P, Dalgaard, P, Juul, A

J Clin Endocrinol Metab 1995
15126567 The role of the acid-labile subunit in regulating insulin-like growth factor transport across human umbilical vein endothelial cell monolayers

Payet, LD, Baxter, RC, Firth, SM

J Clin Endocrinol Metab 2004
9709957 Identification of novel high molecular weight insulin-like growth factor-binding protein-3 association proteins in human serum

Gibson, TB, Collett-Solberg, PF, Nunn, SE, Cohen, P

J Clin Endocrinol Metab 1998
11250653 Ligand-binding characteristics of recombinant amino- and carboxyl-terminal fragments of human insulin-like growth factor-binding protein-3

Bond, J, Kortt, AA, Hudson, PJ, Nathanielsz, A, Galanis, M, Baxter, RC, Firth, SM

J Endocrinol 2001
15469690 Characterization of insulin-like growth factor-free interaction between insulin-like growth factor binding protein 3 and acid labile subunit expressed from Xenopus oocytes

Lee, DH, Lee, CY, Kyung, YJ, Choi, KY

J Biochem Mol Biol 2004
7689487 Glucose does not influence the insulin-like growth factor (JGF) binding to carrier proteins (IGFBPs): analysis of rat and human serum by western ligand blotting

Savage, MO, Holly, JM, Frost, VJ, Cianfarani, S

Experientia 1993
8781553 Measurement of insulin-like growth factor (IGF)-II binding to purified IGF binding proteins 1-6: comparison of charcoal adsorption and high performance size exclusion chromatography

Rechler, MM, Bach, LA

Biochim Biophys Acta 1996
7519608 Heparin, heparan sulfate, and dermatan sulfate regulate formation of the insulin-like growth factor-I and insulin-like growth factor-binding protein complexes

Arai, T, Busby W, Jr, Parker, A, Clemmons, DR

J Biol Chem 1994
11788658 Molecular distribution of IGF binding protein-5 in human serum

Baxter, RC, Firth, SM, Meka, S

J Clin Endocrinol Metab 2002
11316783 Mutagenesis of basic amino acids in the carboxyl-terminal region of insulin-like growth factor binding protein-5 affects acid-labile subunit binding

Baxter, RC, Clemmons, DR, Firth, SM

Endocrinology 2001
12810533 Amino- and carboxyl-terminal fragments of insulin-like growth factor (IGF) binding protein-3 cooperate to bind IGFs with high affinity and inhibit IGF receptor interactions

Payet, LD, Wang, XH, Baxter, RC, Firth, SM

Endocrinology 2003
10810289 Binding characteristics of pro-insulin-like growth factor-II from cancer patients: binary and ternary complex formation with IGF binding proteins-1 to -6

Baxter, RC, Meka, S, Bond, JJ

J Endocrinol 2000
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