TRIM28 SUMOylates TRIM28:ZNF350 with SUMO1

Stable Identifier
Reaction [transition]
Homo sapiens
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The PHD domain of TRIM28 (KAP1) acts as a SUMO E3 ligase to SUMOylate the bromodomain of TRIM28 with SUMO1 (Ivanov et al. 2007, Lee et al. 2007, Li et al. 2007, Mascle et al. 2007, Zeng et al. 2008, Impens et al. 2014). SUMOylation enhances repression of p21 transcription by the TRIM28:ZNF350 (KAP1:ZBRK1) complex. SUMOylated TRIM28 recruits SETDB1 and CHD3 (of the NuRD complex).

Literature References
PubMed ID Title Journal Year
18488044 Structural insights into human KAP1 PHD finger-bromodomain and its role in gene silencing

Yap, KL, Ivanov, AV, Rauscher FJ, 3rd, Wang, X, Plotnikova, O, Mujtaba, S, Zhou, MM, Zeng, L

Nat Struct Mol Biol 2008
17942393 Role for KAP1 serine 824 phosphorylation and sumoylation/desumoylation switch in regulating KAP1-mediated transcriptional repression

Ann, DK, Yen, Y, Shih, HM, Lee, YK, Li, X, Schultz, DC, Jeng, JC

J. Biol. Chem. 2007
17079232 Doxorubicin down-regulates Kruppel-associated box domain-associated protein 1 sumoylation that relieves its transcription repression on p21WAF1/CIP1 in breast cancer MCF-7 cells

Ann, DK, Yang, AJ, Lee, YK, Thomas, SN

J. Biol. Chem. 2007
18082607 PHD domain-mediated E3 ligase activity directs intramolecular sumoylation of an adjacent bromodomain required for gene silencing

Yap, KL, Negorev, DG, Ivanov, AV, Schultz, DC, Peng, H, Fredericks, WJ, Sadofsky, MJ, Psulkowski, E, Rauscher FJ, 3rd, White, DE, Yurchenko, V, Zhou, MM, Maul, GG

Mol Cell 2007
17298944 Sumoylation of the transcriptional intermediary factor 1beta (TIF1beta), the Co-repressor of the KRAB Multifinger proteins, is required for its transcriptional activity and is modulated by the KRAB domain

Huynh, P, Germain-Desprez, D, Aubry, M, Estephan, P, Mascle, XH

J. Biol. Chem. 2007
25114211 Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli

Impens, F, Cossart, P, Radoshevich, L, Ribet, D

Proc. Natl. Acad. Sci. U.S.A. 2014
Catalyst Activity

SUMO transferase activity of TRIM28 [nucleoplasm]

Orthologous Events
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