Reactome | WLS:WNT complex is internalized for maturation into exosomes

WLS:WNT complex is internalized for maturation into exosomes

Stable Identifier

R-HSA-3781943

Type

Reaction [omitted]

Species

Homo sapiens

Compartment

plasma membrane, endocytic vesicle membrane

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WLS:WNT complex is internalized for maturation into exosomes

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Fractionation of extracellular WNT activity shows that between 12-40% of secreted WNT ligand is present on exosomal vesicles (Gross et al, 2012; Beckett et al, 2013). Exosomes are 40 - 100 nm microvesicles of endocytic origin with established roles in cell-cell communication. They are produced by multivesicular bodies (MVBs) and directed to the plasma membrane for secretion (reviewed in Simons and Raposo, 2009). WNT secretion in the exosomal fraction is dependent on WLS/EVI/SPR in both human and Drosophila cells (Gross et al, 2012; Beckett et al, 2013). While exosomes have been shown to be required for presynaptic release of EVI and Wg at Drosophila neuromuscular junctions, there is conflicting evidence about whether they play a role in the formation of a Wg gradient at the Drosophila imaginal disc (Korkut et al, 2009; Gross et al, 2012; Beckett et al, 2013). Exosomal WNT fractions co-purify with TSG101 and other components of the ESCRT machinery, and knockdown of ESCRT 0 components reduces the levels of WNT3A and the signaling activity of the exosomal fractions (Gross et al, 2012; Beckett et al, 2013).

Literature References

PubMed ID	Title	Journal	Year
22983114	Active Wnt proteins are secreted on exosomes Gross, JC, Chaudhary, V, Bartscherer, K, Boutros, M	Nat. Cell Biol.	2012
19442504	Exosomes--vesicular carriers for intercellular communication Simons, M, Raposo, G	Curr. Opin. Cell Biol.	2009
19837038	Trans-synaptic transmission of vesicular Wnt signals through Evi/Wntless Korkut, C, Ataman, B, Ramachandran, P, Ashley, J, Barria, R, Gherbesi, N, Budnik, V	Cell	2009
23260141	Exosomes mediate stromal mobilization of autocrine Wnt-PCP signaling in breast cancer cell migration Luga, V, Zhang, L, Viloria-Petit, AM, Ogunjimi, AA, Inanlou, MR, Chiu, E, Buchanan, M, Hosein, AN, Basik, M, Wrana, JL	Cell	2012