Reactome | Kinetochore capture of astral microtubules

Kinetochore capture of astral microtubules

Stable Identifier

R-HSA-375302

Type

Reaction [binding]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

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Kinetochore capture of astral microtubules

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The human kinetochore, is a complex proteinaceous structure that assembles on centromeric DNA and mediates the association of mitotic chromosomes with spindle microtubules in prometaphase. The molecular composition of the human kinetochore is reviewed in detail in Cheeseman et al., 2008. This complex structure is composed of numerous protein complexes and networks including: the constitutive centromere-associated network (CCAN) containing several sub-networks such as (CENP-H, I, K), (CENP-50/U, O, P, Q, R), the KMN network (containing KNL1, the Mis12 complex, and the Ndc80 complex), the chromosomal passenger complex, the mitotic checkpoint complex, the nucleoporin 107-160 complex and the RZZ complex.
At prometaphase, following breakdown of the nuclear envelope, the kinetochores of condensed chromosomes begin to interact with spindle microtubules. In humans, 15-20 microtubules are bound to each kinetochore (McEwen et al., 2001), and the attachment of 15 microtubules to the kinetochore is shown in this reaction. Recently, it was found that the core kinetochore-microtubule attachment site is within the KMN network and is likely to be formed by two closely apposed low-affinity microtubule-binding sites, one in the Ndc80 complex and a second in KNL1 (Cheeseman et al., 2006).

Literature References

PubMed ID	Title	Journal	Year
17030981	Mapping the assembly pathways that specify formation of the trilaminar kinetochore plates in human cells Jablonski, SA, Liu, ST, Yen, TJ, Rattner, JB	J Cell Biol	2006
16622420	The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres Okada, M, McLeod, IX, Hori, T, Yates, JR 3rd, Cheeseman, IM, Okawa, K, Fukagawa, T, Desai, A	Nat Cell Biol	2006
16622419	The human CENP-A centromeric nucleosome-associated complex Jansen, LE, Yates, JR 3rd, Foltz, DR, Black, BE, Bailey, AO, Cleveland, DW	Nat Cell Biol	2006
18097444	Molecular architecture of the kinetochore-microtubule interface Cheeseman, IM, Desai, A	Nat Rev Mol Cell Biol	2008
17129783	The conserved KMN network constitutes the core microtubule-binding site of the kinetochore Cheeseman, IM, Chappie, JS, Wilson-Kubalek, EM, Desai, A	Cell	2006