Tankyrase binds AXIN

Stable Identifier
Reaction [binding]
Homo sapiens
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Several recent chemical screens have identified inhibitors of the poly-ADP ribosylation enzymes tankyrase (TNKS) 1 and 2 as regulators of WNT signalling (Huang et al, 2009; Chen et al, 2009; Waaler et al, 2012). Endogenous TNKS1 and 2 associate with AXIN2 in SW480 cells as assessed by co-immunoprecipitation. Both AXIN1 and AXIN2 interact strongly with TNKS1/2 by two-hybrid, and deletion analysis shows that amino acids 19-30 of AXIN1 are necessary and sufficient for binding to TNKS1. This region, termed the tankyrase-binding-domain (TBD) is necessary and sufficient for the interaction in GST-pulldown and co-immunoprecipitation studies (Huang et al, 2009).

Literature References
PubMed ID Title Journal Year
19125156 Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer

Williams, NS, Fan, CW, Chen, C, Kilgore, J, Amatruda, JF, Wei, S, Dodge, ME, Lu, J, Lum, L, Roth, MG, Ma, Z, Chen, B, Tang, W, Hao, W

Nat. Chem. Biol. 2009
19759537 Tankyrase inhibition stabilizes axin and antagonizes Wnt signalling

Mickanin, C, Zhang, Y, Fawell, S, Mishina, YM, Fazal, A, Myer, V, Wiellette, E, Michaud, GA, Wiessner, S, Hild, M, Bauer, A, Porter, JA, Rau, C, Huang, SM, Lengauer, C, Finan, PM, Ghidelli, S, Serluca, F, Shao, W, Bouwmeester, T, Shi, X, Stegmeier, F, Shultz, M, Charlat, O, Kirschner, MW, Cong, F, Schirle, M, Schlegl, J, Curtis, D, Wilson, CJ, Tallarico, JA, Cheng, H, Cheung, A, Liu, S, Lu, C, Tomlinson, R

Nature 2009
22440753 A novel tankyrase inhibitor decreases canonical Wnt signaling in colon carcinoma cells and reduces tumor growth in conditional APC mutant mice

Wilson, SR, Machon, O, Korinek, V, Waaler, J, Dinh, H, Machonova, O, Krauss, S, Eide, TJ, Tumova, L, Voronkov, A, von Kries, JP, Paulsen, JE, Gradl, D, Pedersen, NM

Cancer Res. 2012
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