Reactome | Nuclear Pore Complex (NPC) Disassembly

Nuclear Pore Complex (NPC) Disassembly

Stable Identifier

R-HSA-3301854

Type

Pathway

Species

Homo sapiens

ReviewStatus

5/5

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Nuclear envelope breakdown in mitosis involves permeabilization of the nuclear envelope through disassembly of the nuclear pore complex (NPC) (reviewed by Guttinger et al. 2009). Nucleoporin NUP98, located at both the cytoplasmic and the nucleoplasmic side of the NPC (Griffis et al. 2003), and involved in the formation of the transport barrier through its FG (phenylalanine glycine) repeats that protrude into the central cavity of the NPC (Hulsmann et al. 2012), is probably the first nucleoporin that dissociates from the NPC at the start of mitotic NPC disassembly (Dultz et al. 2008). NUP98 dissociation is triggered by phosphorylation. Phosphorylation of NUP98 by CDK1 and NIMA family kinases NEK6 and/or NEK7 is needed for NUP98 dissociation from the NPC (Laurell et al. 2011). While the phosphorylation of NUP98 by CDK1 and NEK6/7 is likely to occur simultaneously, CDK1 and NEK6/7-mediated phosphorylations are shown as separate events, for clarity purposes.

Literature References

PubMed ID	Title	Journal	Year
22901806	The permeability of reconstituted nuclear pores provides direct evidence for the selective phase model Hülsmann, BB, Labokha, AA, Görlich, D	Cell	2012
21335236	Phosphorylation of Nup98 by multiple kinases is crucial for NPC disassembly during mitotic entry Laurell, E, Beck, K, Krupina, K, Theerthagiri, G, Bodenmiller, B, Horvath, P, Aebersold, R, Antonin, W, Kutay, U	Cell	2011
19234477	Orchestrating nuclear envelope disassembly and reassembly during mitosis Güttinger, S, Laurell, E, Kutay, U	Nat. Rev. Mol. Cell Biol.	2009
12589057	Nup98 localizes to both nuclear and cytoplasmic sides of the nuclear pore and binds to two distinct nucleoporin subcomplexes Griffis, ER, Xu, S, Powers, MA	Mol. Biol. Cell	2003
18316408	Systematic kinetic analysis of mitotic dis- and reassembly of the nuclear pore in living cells Dultz, E, Zanin, E, Wurzenberger, C, Braun, M, Rabut, G, Sironi, L, Ellenberg, J	J. Cell Biol.	2008