SOD2 catalyzes 2H+ + 2O2.- => O2 + H2O2 (mitochondrial matrix)

Stable Identifier
Reaction [transition]
Homo sapiens
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Mn superoxide dismutase (SOD2) is located in the mitochondrial matrix where it catalyzes the reaction of two molecules of superoxide (O2-.) to form one molecule of oxygen (O2) and one molecule of hydrogen peroxide (H2O2). Data from mouse liver indicate that respiratory complex I leaks superoxide into the matrix and respiratory complex III leaks superoxide into both the matrix and the intermembrane space (Muller et al. 2004). Because of its negative charge superoxide is unable to cross membranes, however hydrogen peroxide, a product of SOD2, is released from mitochondria to the cytosol in proportion to the proton potential (inferred from rat heart mitochondria in Boveris et al. 2006, Korshunov et al. 1997).

Literature References
PubMed ID Title Journal Year
2071038 Biochemical and stability properties of recombinant human MnSOD

Werber, MM, Greenstein, LA

Free Radic. Res. Commun. 1991
15317809 Complex III releases superoxide to both sides of the inner mitochondrial membrane

Van Remmen, H, Muller, FL, Liu, Y

J. Biol. Chem. 2004
9369223 High protonic potential actuates a mechanism of production of reactive oxygen species in mitochondria

Starkov, AA, Korshunov, SS, Skulachev, VP

FEBS Lett. 1997
7021796 Purification of superoxide dismutases from human placenta using immunoadsorbent columns

Hirano, K, Inoue, H, Ito, Y, Adachi, T, Sugiura, M

J. Pharmacobio-dyn. 1981
8663465 Catalytic properties of human manganese superoxide dismutase

Nick, HS, O'Connor, D, Hsieh, Y, Silverman, DN, Hsu, JL, Tu, C

J. Biol. Chem. 1996
1668270 A three-step purification of manganese superoxide dismutase from human liver on both large and small scales

Seo, HG, Matsuda, Y, Deutsch, HF, Nakata, T, Kawata, S, Taniguchi, N, Ookawara, T, Tarui, S, Suzuki, K

Protein Expr. Purif. 1991
16615992 Mitochondrial metabolic states regulate nitric oxide and hydrogen peroxide diffusion to the cytosol

Zaobornyj, T, Boveris, A, Valdez, LB, Bustamante, J

Biochim. Biophys. Acta 2006
11912930 Catalytic pathway of manganese superoxide dismutase by direct observation of superoxide

Nick, HS, Silverman, DN

Meth. Enzymol. 2002
Catalyst Activity

superoxide dismutase activity of SOD2 tetramer [mitochondrial matrix]

Orthologous Events
Cross References
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