WLS binds WNT ligands in the Golgi

Stable Identifier
R-HSA-3247840
Type
Reaction [binding]
Species
Homo sapiens
Compartment
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Wntless (WLS) (Evi/Sprinter/GPR177) is a conserved transmembrane protein that is required for the secretion of WNT ligands from the cell (Bänzinger et al, 2006; Bartscherer et al, 2006; Goodman et al, 2006). Notably, WLS is not required for the secretion of Hedgehog, another acylated signaling molecule, and wls-mutants phenocopy wg/wnt mutants (Bänziger et al, 2006; Bartscherer et al, 2006; Goodman et al, 2006), supporting the notion that WLS is a dedicated WNT pathway member. WLS binds directly to WNT ligands in the Golgi in a WNT-acylation dependent manner, as the interaction is abrogated by mutation of either PORCN or the conserved Ser209 residue (Coombs et al, 2010; Herr and Basler, 2012). WLS is thought to contain a lipocalin-family fold (Coombs et al, 2010), a lipid-interacting domain, which may play a role in binding to the lipid adduct on WNT.

Literature References
PubMed ID Title Journal Year
20826466 WLS-dependent secretion of WNT3A requires Ser209 acylation and vacuolar acidification

Coombs, GS, Yu, J, Canning, CA, Veltri, CA, Covey, TM, Cheong, JK, Utomo, V, Banerjee, N, Zhang, ZH, Jadulco, RC, Concepcion, GP, Bugni, TS, Harper, MK, Mihalek, I, Jones, CM, Ireland, CM, Virshup, DM

J. Cell. Sci. 2010
22108505 Porcupine-mediated lipidation is required for Wnt recognition by Wls

Herr, P, Basler, K

Dev. Biol. 2012
16678096 Secretion of Wnt ligands requires Evi, a conserved transmembrane protein

Bartscherer, K, Pelte, N, Ingelfinger, D, Boutros, M

Cell 2006
17108000 Sprinter: a novel transmembrane protein required for Wg secretion and signaling

Goodman, RM, Thombre, S, Firtina, Z, Gray, D, Betts, D, Roebuck, J, Spana, EP, Selva, EM

Development 2006
16678095 Wntless, a conserved membrane protein dedicated to the secretion of Wnt proteins from signaling cells

Bänziger, C, Soldini, D, Schütt, C, Zipperlen, P, Hausmann, G, Basler, K

Cell 2006
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