Reactome | WLS binds WNT ligands in the Golgi

WLS binds WNT ligands in the Golgi

Stable Identifier

R-HSA-3247840

Type

Reaction [binding]

Species

Homo sapiens

Compartment

Golgi membrane, Golgi lumen

ReviewStatus

5/5

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Wntless (WLS) (Evi/Sprinter/GPR177) is a conserved transmembrane protein that is required for the secretion of WNT ligands from the cell (Bänzinger et al, 2006; Bartscherer et al, 2006; Goodman et al, 2006). Notably, WLS is not required for the secretion of Hedgehog, another acylated signaling molecule, and wls-mutants phenocopy wg/wnt mutants (Bänziger et al, 2006; Bartscherer et al, 2006; Goodman et al, 2006), supporting the notion that WLS is a dedicated WNT pathway member. WLS binds directly to WNT ligands in the Golgi in a WNT-acylation dependent manner, as the interaction is abrogated by mutation of either PORCN or the conserved Ser209 residue (Coombs et al, 2010; Herr and Basler, 2012). WLS is thought to contain a lipocalin-family fold (Coombs et al, 2010), a lipid-interacting domain, which may play a role in binding to the lipid adduct on WNT.

Literature References

PubMed ID	Title	Journal	Year
22108505	Porcupine-mediated lipidation is required for Wnt recognition by Wls Herr, P, Basler, K	Dev. Biol.	2012
20826466	WLS-dependent secretion of WNT3A requires Ser209 acylation and vacuolar acidification Coombs, GS, Utomo, V, Jones, CM, Bugni, TS, Cheong, JK, Zhang, ZH, Harper, MK, Virshup, DM, Veltri, CA, Jadulco, RC, Banerjee, N, Concepcion, GP, Yu, J, Mihalek, I, Canning, CA, Ireland, CM, Covey, TM	J. Cell. Sci.	2010
16678096	Secretion of Wnt ligands requires Evi, a conserved transmembrane protein Bartscherer, K, Boutros, M, Pelte, N, Ingelfinger, D	Cell	2006
17108000	Sprinter: a novel transmembrane protein required for Wg secretion and signaling Firtina, Z, Spana, EP, Betts, D, Thombre, S, Selva, EM, Goodman, RM, Roebuck, J, Gray, D	Development	2006
16678095	Wntless, a conserved membrane protein dedicated to the secretion of Wnt proteins from signaling cells Zipperlen, P, Soldini, D, Bänziger, C, Hausmann, G, Schütt, C, Basler, K	Cell	2006