MTRR reduces cob(II)alamin to meCbl

Stable Identifier
Reaction [transition]
Homo sapiens
2 (methionine synthase)-cob(II)alamin + NADPH + 2 S-adenosyl-L-methionine => 2 (methionine synthase)-methylcob(III)alamin + H(+) + NADP(+) + 2 S-adenosyl-L-homocysteine
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Methionine synthase reductase (MTRR), in a stable complex with methionine synthase (MTR), catalyzes the reduction of cob(II)alamin to methylcobalamin (MeCbl), the cofactor form required for MTR activity. This reductive methylation reaction uses S adenosylmethionine (AdoMet, SAM) as a methyl donor. MTRR requires 1 FMN and 1 FAD per subunit for activity (Wolthers & Scrutton 2007, Wolthers & Scrutton 2009).

An important role for this reaction in the cell is maintenance of the pool of MeCbl-associated MTR. At a low rate, cobalamin bound to MTR is spontaneously oxidized to form cob(II)alamin. When this happens, MTRR this reductive methylation reaction restores cob(II)alamin to MeCbl (Hall et al. 2000).

Defects in MTRR cause methylcobalamin deficiency type E (cblE; MIM:236270) (Wilson et al. 1999). Patients with cblE exhibit megaloblastic anemia and hyperhomocysteinemia. AdoMet is used as a methyl donor in many biological reactions and its demethylation produces homocysteine. Remethylation is carried out by MTR in conjunction with MTRR but in cblE patients, MTR bound cobalamin cannot be reduced by defective MTRR to form a functional enzyme thus homocysteine accumulates. Mutations in MTRR that cause cblE include Leu576del (Leclerc et al. 1998) and S454L (Zavadakova et al. 2005). In terms of frequency, the most common MTRR mutation is a c.903+469C>T mutation which creates a novel splice site deep in an intron and results in inclusion of a 140 bp insertion in MTRR mRNA (Homolova et al. 2010). Wilson et al. showed that a 66A G polymorphism, resulting in an Ile22Met (I22M) substitution, is associated with susceptibility to folate sensitive neural tube defects (FS NTD; MIM:601634) (Wilson et al. 1999b, Doolin et al. 2002). Serum deficiency of vitamin B12 increased the effect.

Literature References
PubMed ID Title Journal Year
12375236 Maternal genetic effects, exerted by genes involved in homocysteine remethylation, influence the risk of spina bifida

Mitchell, LE, Whitehead, AS, McDonnell, M, Hoess, K, Doolin, MT, Barbaux, S

Am. J. Hum. Genet. 2002
17892308 Mechanism of coenzyme binding to human methionine synthase reductase revealed through the crystal structure of the FNR-like module and isothermal titration calorimetry

Scrutton, NS, Wolthers, KR, Leys, D, Lou, X, Toogood, HS

Biochemistry 2007
10484769 Molecular basis for methionine synthase reductase deficiency in patients belonging to the cblE complementation group of disorders in folate/cobalamin metabolism

Gravel, RA, Wilson, A, Leclerc, D, Rosenblatt, DS

Hum. Mol. Genet. 1999
19243433 Cobalamin uptake and reactivation occurs through specific protein interactions in the methionine synthase-methionine synthase reductase complex

Scrutton, NS, Wolthers, KR

FEBS J. 2009
10978155 Interaction of flavodoxin with cobalamin-dependent methionine synthase

Jordan-Starck, TC, Ludwig, ML, Loo, RO, Hall, DA, Matthews, RG

Biochemistry 2000
10444342 A common variant in methionine synthase reductase combined with low cobalamin (vitamin B12) increases risk for spina bifida

Gravel, RA, Yang, H, Rozen, R, Wilson, A, Christensen, B, Leclerc, D, Wu, Q, Platt, R

Mol. Genet. Metab. 1999
17477549 Protein interactions in the human methionine synthase-methionine synthase reductase complex and implications for the mechanism of enzyme reactivation

Scrutton, NS, Wolthers, KR

Biochemistry 2007
Catalyst Activity

[methionine synthase] reductase activity of MTRR:MTR:cob(II)alamin [cytosol]

Orthologous Events
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