VRK1/VRK2 phosphorylate BANF1

Stable Identifier
R-HSA-2993898
Type
Reaction [transition]
Species
Homo sapiens
Compartment
nuclear envelope, nucleoplasm, chromosome
Locations in the PathwayBrowser
Expand all
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
VRK1/VRK2 phosphorylate BANF1
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

In mitotic prophase, chromatin detaches from the nuclear envelope, and this contributes to the nuclear envelope breakdown. VRK1 (and possibly VRK2) mediated phosphorylation of BANF1 (BAF), a protein that simultaneously interacts with DNA, LEM-domain inner nuclear membrane proteins, and lamins (Zheng et al. 2000, Shumaker et al. 2001, Haraguchi et al. 2001, Mansharamani and Wilson 2005, Brachner et al. 2005) is considered to be one of the key steps in the detachment of the nuclear envelope from chromatin (Bengtsson and Wilson 2006, Nichols et al. 2006, Gorjanacz et al. 2007).

BANF1 (BAF i.e. barrier-to-autointegration factor) is a DNA-binding protein that was initially discovered as a regulator of retroviral integration (Lee and Craigie 1994, Lee and Craigie 1998). BANF1 (BAF) binds DNA non-specifically as a homodimer (Zheng et al. 2000). Proteins of the inner nuclear membrane that possess a LEM domain, TMPO (LAP2beta), EMD (emerin), LEMD3 (MAN1) and LEMD2 (LEM2), form three-way complexes with BANF1 and lamins - intermediary filaments of the nucleoplasm (Shumaker et al. 2001, Holaska et al. 2003, Mansharamani and Wilson 2005, Brachner et al. 2005). These complexes are thought to be important for the structure of the nuclear lamina and also enable attachment of chromatin to the nuclear envelope (Haraguchi et al. 2001, Dechat et al. 2004).

In mitosis, VRK1 (and to a lesser extent VRK2) serine/threonine kinase phosphorylates BANF1 (BAF) on serine residue S4 and threonine residues T2 and T3 (Nichols et al. 2006, Gorjanacz et al. 2007, Asencio et al. 2012). Only VRK2 isoform VRK2-2 which can localize to the nucleus (Blanco et al. 2006) is annotated as BANF1 kinase. Phosphorylated BANF1 (BAF) dissociates from chromatin and the inner nuclear membrane proteins (Bengtsson and Wilson 2006), allowing chromatin to detach from the nuclear envelope.

VRK1 and VRK2 are autophosphorylated but not all autophosphorylation sites have been mapped and the impact of autophosphorylation on catalytic activity has not been determined.

Literature References
PubMed ID Title Journal Year
11285238 LAP2 binds to BAF.DNA complexes: requirement for the LEM domain and modulation by variable regions

Shumaker, DK, Lee, KK, Tanhehco, YC, Craigie, R, Wilson, KL

EMBO J. 2001
12493765 Transcriptional repressor germ cell-less (GCL) and barrier to autointegration factor (BAF) compete for binding to emerin in vitro

Holaska, JM, Lee, KK, Kowalski, AK, Wilson, KL

J. Biol. Chem. 2003
16495336 The vaccinia-related kinases phosphorylate the N' terminus of BAF, regulating its interaction with DNA and its retention in the nucleus

Nichols, RJ, Wiebe, MS, Traktman, P

Mol. Biol. Cell 2006
16704422 The subcellular localization of vaccinia-related kinase-2 (VRK2) isoforms determines their different effect on p53 stability in tumour cell lines

Blanco, S, Klimcakova, L, Vega, FM, Lazo, PA

FEBS J. 2006
7937898 Protection of retroviral DNA from autointegration: involvement of a cellular factor

Lee, MS, Craigie, R

Proc Natl Acad Sci U S A 1994
22770216 Coordination of kinase and phosphatase activities by Lem4 enables nuclear envelope reassembly during mitosis

Asencio, C, Davidson, IF, Santarella-Mellwig, R, Ly-Hartig, TB, Mall, M, Wallenfang, MR, Mattaj, IW, Gorjánácz, M

Cell 2012
15681850 Direct binding of nuclear membrane protein MAN1 to emerin in vitro and two modes of binding to barrier-to-autointegration factor

Mansharamani, M, Wilson, KL

J. Biol. Chem. 2005
11792822 BAF is required for emerin assembly into the reforming nuclear envelope

Haraguchi, T, Koujin, T, Segura-Totten, M, Lee, KK, Matsuoka, Y, Yoneda, Y, Wilson, KL, Hiraoka, Y

J. Cell. Sci. 2001
16339967 LEM2 is a novel MAN1-related inner nuclear membrane protein associated with A-type lamins

Brachner, A, Reipert, S, Foisner, R, Gotzmann, J

J. Cell. Sci. 2005
10908652 Barrier-to-autointegration factor (BAF) bridges DNA in a discrete, higher-order nucleoprotein complex

Zheng, R, Ghirlando, R, Lee, MS, Mizuuchi, K, Krause, M, Craigie, R

Proc. Natl. Acad. Sci. U.S.A. 2000
9465049 A previously unidentified host protein protects retroviral DNA from autointegration

Lee, MS, Craigie, R

Proc Natl Acad Sci U S A 1998
17170708 Caenorhabditis elegans BAF-1 and its kinase VRK-1 participate directly in post-mitotic nuclear envelope assembly

Gorjánácz, M, Klerkx, EP, Galy, V, Santarella, R, López-Iglesias, C, Askjaer, P, Mattaj, IW

EMBO J. 2007
16371512 Barrier-to-autointegration factor phosphorylation on Ser-4 regulates emerin binding to lamin A in vitro and emerin localization in vivo

Bengtsson, L, Wilson, KL

Mol. Biol. Cell 2006
15546916 LAP2alpha and BAF transiently localize to telomeres and specific regions on chromatin during nuclear assembly

Dechat, T, Gajewski, A, Korbei, B, Gerlich, D, Daigle, N, Haraguchi, T, Furukawa, K, Ellenberg, J, Foisner, R

J. Cell. Sci. 2004
Participants
Input
Output
Participant Of
hasEvent
Catalyst Activity
Catalyst Activity
Title
protein serine/threonine kinase activity of VRK1/VRK2 [nucleoplasm]
Physical Entity
VRK1/VRK2 [nucleoplasm]
Activity
protein serine/threonine kinase activity (0004674)
Orthologous Events
VRK1/VRK2 phosphorylate BANF1 (Bos taurus)
VRK1/VRK2 phosphorylate BANF1 (Canis familiaris)
VRK1/VRK2 phosphorylate BANF1 (Mus musculus)
VRK1/VRK2 phosphorylate BANF1 (Rattus norvegicus)
VRK1/VRK2 phosphorylate BANF1 (Sus scrofa)
VRK1/VRK2 phosphorylate BANF1 (Xenopus tropicalis)
Authored
Orlic-Milacic, M  (2013-01-23)
Reviewed
Gorjánácz, M  (2013-01-30)
Mattaj, IW  (2013-01-30)
Created
Orlic-Milacic, M  (2013-01-21)