Reactome | CDK1 phosphorylates NUP98

CDK1 phosphorylates NUP98

Stable Identifier

R-HSA-2990882

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol, nuclear envelope

ReviewStatus

5/5

Locations in the PathwayBrowser

Expand all

Cell Cycle (Homo sapiens)

- Cell Cycle, Mitotic (Homo sapiens)
  - M Phase (Homo sapiens)
    - Mitotic Prophase (Homo sapiens)
      - Nuclear Envelope Breakdown (Homo sapiens)
        
        Nuclear Pore Complex (NPC) Disassembly (Homo sapiens)
        
        CDK1 phosphorylates NUP98 (Homo sapiens)

General

SBML | | PDF

SVG | | PPTX | SBGN

Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

CDK1 activity promotes the nuclear pore complex (NPC) disassembly in mitosis (Muhlhausser and Kutay 2007). While NUP98 is probably not the only nucleoporin phosphorylated by CDK1 at mitotic entry, NUP98 is the best characterized CDK1 target among nuclear pore complex components. NUP98 threonine residues T529, T536, and T653, as well as serine residues S595 and S606 were found to be phosphorylated when NUP98 was isolated from mitotic HeLa cells (human cervical carcinoma cell line); these five sites match the CDK1 target site consensus and are phosphorylated by CDK1:CCNB in vitro (Laurell et al. 2011). The NUP98 splicing isoform NUP98-4 was used in the study by Laurell et al. 2011 and the indicated positions of phosphorylated amino acid residues refer to this isoform. An additional splicing isoform NUP98-3, the product of an alternative splicing site in exon10 of the NUP98 gene, which is 17 amino acids longer than NUP98-4, could also be a part of the NPC. CDK1-phosphorylated residues in NUP98-3 would be threonines T546, T553 and T670, and serines S612 and S623.

Literature References

PubMed ID	Title	Journal	Year
17698605	An in vitro nuclear disassembly system reveals a role for the RanGTPase system and microtubule-dependent steps in nuclear envelope breakdown Mühlhäusser, P, Kutay, U	J. Cell Biol.	2007
21335236	Phosphorylation of Nup98 by multiple kinases is crucial for NPC disassembly during mitotic entry Aebersold, R, Beck, K, Antonin, W, Krupina, K, Horvath, P, Bodenmiller, B, Theerthagiri, G, Laurell, E, Kutay, U	Cell	2011