Reactome | PLK1 phosphorylates NEK9

PLK1 phosphorylates NEK9

Stable Identifier

R-HSA-2984226

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

Locations in the PathwayBrowser

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Cell Cycle (Homo sapiens)

- Cell Cycle, Mitotic (Homo sapiens)
  - M Phase (Homo sapiens)
    - Mitotic Prophase (Homo sapiens)
      - Nuclear Envelope Breakdown (Homo sapiens)
        
        Activation of NIMA Kinases NEK9, NEK6, NEK7 (Homo sapiens)
        
        PLK1 phosphorylates NEK9 (Homo sapiens)

General

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NEK9 serine residues S29, S750 and S869, which are likely targets of CDK1:CCNB-mediated phosphorylation in mitosis, can be recognized by the polo-box domain (PBD) of PLK1 when phosphorylated. Phosphorylation of S869 appears to be crucial for the interaction of NEK9 and PLK1 (Bertran et al. 2011). PLK1 phosphorylates threonine T210 of NEK9 in vitro. T210 is located in the kinase activation loop of NEK9 and T210 phosphorylation is necessary for NEK9 kinase activity. While T210 can be autophosphorylated in vitro, when NEK9 is incubated in the presence of excess ATP and Mg2+ (Roig et al. 2005), mitotic phosphorylation of T210 requires both CDK1 and PLK1 activity (Bertran et al. 2011).

Literature References

PubMed ID	Title	Journal	Year
16079175	Active Nercc1 protein kinase concentrates at centrosomes early in mitosis and is necessary for proper spindle assembly Roig, J, Groen, A, Caldwell, J, Avruch, J	Mol. Biol. Cell	2005
21642957	Nek9 is a Plk1-activated kinase that controls early centrosome separation through Nek6/7 and Eg5 Bertran, MT, Sdelci, S, Regué, L, Avruch, J, Caelles, C, Roig, J	EMBO J.	2011