CDK1 phosphorylates condensin I

Stable Identifier
R-HSA-2514854
Type
Reaction [transition]
Species
Homo sapiens
Compartment
cytosol
ReviewStatus
5/5
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CDK1 phosphorylates condensin I
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CDK1 (CDC2) in complex with CCNB (cyclin B) phosphorylates condensin I subunits NCAPD2, NCAPG and NCAPH in mitosis (Kimura et al. 2001, Takemoto et al. 2006), but other mitotic kinases may also be involved. CDK1 phosphorylation sites in NCAPH have not been established. NCAPD2 threonine residues T1339, T1384 and T1389 are inferred to be phosphorylated by CDK1 based on homologues sites in Xenopus laevis Ncapd2 (Kimura et al. 1998). NCAPG threonine residues T308 and T332 are phosphorylated by CDK1 in vitro and functionally important. The functional importance of threonine T931, also phosphorylated by CDK1 in vitro, has not been demonstrated (Murphy et al. 2008). Phosphorylation by CDK1 is required for mitotic activation of condensin I and promotes chromosomal binding, introduction of positive supercoils into DNA, and chromatin condensation (Kimura et al. 1998, Kimura et al. 2001, Takemoto et al. 2006).
Literature References
PubMed ID Title Journal Year
11136719 Chromosome condensation by a human condensin complex in Xenopus egg extracts

Kimura, K, Cuvier, O, Hirano, T

J. Biol. Chem. 2001
18977199 Phosphorylation of CAP-G is required for its chromosomal DNA localization during mitosis

Murphy, LA, Sarge, KD

Biochem. Biophys. Res. Commun. 2008
9774278 Phosphorylation and activation of 13S condensin by Cdc2 in vitro

Kimura, K, Hirano, M, Kobayashi, R, Hirano, T

Science 1998
17066080 Negative regulation of condensin I by CK2-mediated phosphorylation

Takemoto, A, Kimura, K, Yanagisawa, J, Yokoyama, S, Hanaoka, F

EMBO J. 2006
Participants
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Output
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as an event of
Catalyst Activity

cyclin-dependent protein serine/threonine kinase activity of CCNB1,CCNB2:p-T161-CDK1 [cytosol]

Physical Entity
CCNB1,CCNB2:p-T161-CDK1 [cytosol] (Homo sapiens)
Activity
cyclin-dependent protein serine/threonine kinase activity (GO:0004693)
Inferred From
Cdk1 phosphorylates condensin I (Xenopus laevis)
Authored
Orlic-Milacic, M  (2012-10-12)
Reviewed
Created
Orlic-Milacic, M  (2012-10-10)
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