Reactome | Cohesin Loading onto Chromatin

Cohesin Loading onto Chromatin

Stable Identifier

R-HSA-2470946

Type

Pathway

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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In mitotic telophase, as chromosomes decondense, cohesin complex associated with PDS5 (PDS5A and PDS5B) and WAPAL (WAPL) proteins is loaded onto chromatin (Shintomi and Hirano, 2009, Kueng et al. 2006, Gandhi et al. 2006, Chan et al. 2012). Cohesin loading is facilitated by the complex of NIPBL (SCC2) and MAU2 (SCC4) proteins, which constitute an evolutionarily conserved cohesin loading complex. MAU2 depletion in HeLa cells results in 2-3-fold reduction in the amount of cohesin in the chromatin fraction (Watrin et al. 2006). NIPBL mutations are the cause of the Cornelia de Lange syndrome, a dominantly inherited disorder characterized by facial malformations, limb defects, and growth and cognitive retardation (Tonkin et al. 2004). Cornelia de Lange syndrome can also be caused by mutations in cohesin subunits SMC1A (Musio et al. 2006, Borck et al. 2007, Deardorff et al. 2007, Pie et al. 2010) and SMC3 (Deardorff et al. 2007).

Literature References

PubMed ID	Title	Journal	Year
17113138	Wapl controls the dynamic association of cohesin with chromatin Peters, BH, Hegemann, B, Peters, JM, Mechtler, K, Schleiffer, A, Kueng, S, Lipp, JJ	Cell	2006
16604071	X-linked Cornelia de Lange syndrome owing to SMC1L1 mutations Focarelli, ML, Milani, D, Musio, A, Selicorni, A, Russo, S, Larizza, L, Vezzoni, P, Gervasini, C	Nat. Genet.	2006
19696148	Releasing cohesin from chromosome arms in early mitosis: opposing actions of Wapl-Pds5 and Sgo1 Shintomi, K, Hirano, T	Genes Dev.	2009
16682347	Human Scc4 is required for cohesin binding to chromatin, sister-chromatid cohesion, and mitotic progression Nasmyth, K, Peters, JM, Tanaka, K, Schleiffer, A, Watrin, E, Eisenhaber, F	Curr. Biol.	2006
20358602	Mutations and variants in the cohesion factor genes NIPBL, SMC1A, and SMC3 in a cohort of 30 unrelated patients with Cornelia de Lange syndrome López-Viñas, E, Krantz, ID, Casals, N, Hegardt, FG, Gil-Rodríguez, MC, Deardorff, MA, Rubio, E, Arnedo, M, Ribate, MP, Baldellou, A, Legarreta, J, Pie, J, Olivares, JL, Gomez-Puertas, P, Calvo, MT, Bueno, I, Losada, A, de Karam, JC, Puisac, B, Ramos, FJ, Ciero, M	Am. J. Med. Genet. A	2010
17273969	Mutations in cohesin complex members SMC3 and SMC1A cause a mild variant of cornelia de Lange syndrome with predominant mental retardation Krantz, ID, Wilson, M, Lillquist, K, Gil-Rodríguez, C, Deardorff, MA, Loeys, B, Arnedo, M, Siu, V, Dorsett, D, Pie, J, Kaur, M, Yaeger, D, Musio, A, Rampuria, A, Kline, AD, Jackson, LS, Ramos, FJ, Korolev, S	Am. J. Hum. Genet.	2007
17112726	Human Wapl is a cohesin-binding protein that promotes sister-chromatid resolution in mitotic prophase Gandhi, R, Hirano, T, Gillespie, PJ	Curr. Biol.	2006
17221863	Incidence and clinical features of X-linked Cornelia de Lange syndrome due to SMC1L1 mutations Borck, G, Munnich, A, Bonnefont, JP, Zarhrate, M, Cormier-Daire, V, Colleaux, L	Hum. Mutat.	2007
22901742	Cohesin's DNA Exit Gate Is Distinct from Its Entrance Gate and Is Regulated by Acetylation Roig, MB, Nasmyth, K, Beckouët, F, Chan, KL, Metson, J, Hu, B	Cell	2012
15146185	NIPBL, encoding a homolog of fungal Scc2-type sister chromatid cohesion proteins and fly Nipped-B, is mutated in Cornelia de Lange syndrome Tonkin, ET, Bamshad, MJ, Strachan, T, Lisgo, S, Wang, TJ	Nat. Genet.	2004