MAPK3-3 or MAPK1 phosphorylate GORASP2

Stable Identifier
R-HSA-2422927
Type
Reaction [transition]
Species
Homo sapiens
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GORASP2 (GRASP55) localizes to the median region of Golgi, where it forms a complex with BLZF1 (Golgin 45) and RAB2A GTPase (Short et al. 2001). Similar to GORASP1, GORASP2 is involved in the maintenance of Golgi structure and positively regulates stacking of Golgi cisternae (Xiang and Wang 2010). In addition, GORASP2, probably through its association with RAB2A GTPase, regulates trafficking through the Golgi (Short et al. 2001). In G2 and mitotic prophase, GORASP2 is phosphorylated by MEK1/2 activated MAP kinases. Monophosphorylated MAPK3 (ERK1) isoform, MAPK3 3 i.e. ERK1b (known as ERK1c in rat), likely activated by a MEK1 isoform MEK1b (Shaul et al. 2009), as well as MAPK1 (ERK2) are implicated in GORASP2 phosphorylation during mitosis (Jesch et al. 2001, Colanzi et al. 2003, Shaul and Seger 2006, Feinstein and Linstedt 2007, Duran et al. 2008, Feinstein and Linstedt 2008). Threonine residues T222 and T225 were implicated as targets of MAPK mediated GORASP2 phosphorylation in studies that used directional mutagenesis (Jesch et al. 2001, Feinstein and Linstedt 2008). However both T222 and T225 were simultaneously mutated in these studies and their roles have not been individually investigated. Using mass spectroscopy, T225 but not T222 was identified as a GORASP2 residue phosphorylated by mitotic cytosol (Duran et al. 2008). T249 residue of GORASP2 was also phosphorylated by mitotic cytosol, but the involvement of ERKs in T249 phosphorylation has not been examined (Duran et al. 2008).

Literature References
PubMed ID Title Journal Year
19651986 Specific phosphorylation and activation of ERK1c by MEK1b: a unique route in the ERK cascade

Shaul, YD, Gibor, G, Plotnikov, A, Seger, R

Genes Dev. 2009
16533948 ERK1c regulates Golgi fragmentation during mitosis

Shaul, YD, Seger, R

J. Cell Biol. 2006
17182854 Mitogen-activated protein kinase kinase 1-dependent Golgi unlinking occurs in G2 phase and promotes the G2/M cell cycle transition

Feinstein, TN, Linstedt, AD

Mol. Biol. Cell 2007
11739401 A GRASP55-rab2 effector complex linking Golgi structure to membrane traffic

Short, B, Preisinger, C, K├Ârner, R, Kopajtich, R, Byron, O, Barr, FA

J. Cell Biol. 2001
18434598 GRASP55 regulates Golgi ribbon formation

Feinstein, TN, Linstedt, AD

Mol. Biol. Cell 2008
12695496 RAF1-activated MEK1 is found on the Golgi apparatus in late prophase and is required for Golgi complex fragmentation in mitosis

Colanzi, A, Sutterlin, C, Malhotra, V

J. Cell Biol. 2003
20083603 GRASP55 and GRASP65 play complementary and essential roles in Golgi cisternal stacking

Xiang, Y, Wang, Y

J. Cell Biol. 2010
18385516 The role of GRASP55 in Golgi fragmentation and entry of cells into mitosis

Duran, JM, Kinseth, M, Bossard, C, Rose, DW, Polishchuk, R, Wu, CC, Yates, J, Zimmerman, T, Malhotra, V

Mol. Biol. Cell 2008
11408587 Mitotic phosphorylation of Golgi reassembly stacking protein 55 by mitogen-activated protein kinase ERK2

Jesch, SA, Lewis, TS, Ahn, NG, Linstedt, AD

Mol. Biol. Cell 2001
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Title
protein serine/threonine kinase activity of p-Y204-MAPK3-3/p-T185,Y187-MAPK1 homodimer [cytosol]
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