NUDT1 hydrolyses 2-oxo-ATP to 2-oxo-AMP

Stable Identifier
Reaction [transition]
Homo sapiens
2-hydroxy-ATP + H2O => 2-hydroxy-AMP + PPi
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NUDT1 (MTH1) catalyzes the reaction of 2-oxo-ATP and water to form 2-oxo-AMP and PPi (pyrophosphate). Four NUDT1 proteins have been identified, encoded by a single gene with alternative start codons (Oda et al. 1999). The shortest of these, NUDT1 p18, has been shown to catalyze hydrolysis of 2-oxo-ATP (Fujikawa et al. 2001). The active enzyme is a monomer associated with a magnesium ion (Mishima et al. 2004). The longer isoforms all consist of the p18 polypeptide with aminoterminal extensions and are presumed to be active as well, but have not been experimentally characterized. The p18 isoform is predominantly cytosolic (Kang et al. 1995).

Literature References
PubMed ID Title Journal Year
15133035 Structure of human MTH1, a Nudix family hydrolase that selectively degrades oxidized purine nucleoside triphosphates

Mishima, M, Shirakawa, M, Itoh, N, Takahashi, M, Furuichi, M, Iwai, S, Nakabeppu, Y, Yamagata, Y, Sakai, Y, Kamiya, H

J. Biol. Chem. 2004
11139615 Human MTH1 protein hydrolyzes the oxidized ribonucleotide, 2-hydroxy-ATP

Fujikawa, K, Nakabeppu, Y, Kasai, H, Yakushiji, H, Kamiya, H

Nucleic Acids Res. 2001
7782328 Intracellular localization of 8-oxo-dGTPase in human cells, with special reference to the role of the enzyme in mitochondria

Iyama, A, Furuichi, M, Fujiwara, T, Nishida, J, Nakabeppu, Y, Kang, D, Sekiguchi, M, Takeshige, K

J. Biol. Chem. 1995
Catalyst Activity

2-hydroxy-ATP hydrolase activity of NUDT1 [cytosol]

Orthologous Events
Cross References
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