Reactome | GOLGA2 phosphorylated by CDK1 is unable to promote fusion of ER to Golgi transport vesicles with cis-Golgi

GOLGA2 phosphorylated by CDK1 is unable to promote fusion of ER to Golgi transport vesicles with cis-Golgi

Stable Identifier

R-HSA-2314569

Type

Reaction [omitted]

Species

Homo sapiens

Compartment

ER to Golgi transport vesicle membrane, Golgi membrane

ReviewStatus

5/5

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GOLGA2 phosphorylated by CDK1 is unable to promote fusion of ER to Golgi transport vesicles with cis-Golgi

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USO1 (p115) protein, localizing to membranes of ER to Golgi transport vesicles, binds GOLGA2 (GM130), localizing to membranes of cis-Golgi cisternae. Binding of USO1 to GOLGA2 enables tethering of ER to Golgi transport vesicles to cis-Golgi cisternae, and is facilitated by a Ras-related GTPase RAB1. Fusion of ER to Golgi transport vesicles with cis-Golgi succeeds tethering and depends on STX5 (syntaxin-5). In mitosis, phosphorylation of GOLGA2 by cyclin B-activated CDK1 prevents USO1 docking. This results in cessation of ER to Golgi transport. Halting ER to Golgi transport increases the number of transport vesicles at the expense of Golgi cisternae, since transport vesicles keep budding from the ER but are unable to fuse with Golgi cisternae and deliver their content (Lowe et al. 1998, Seeman et al. 2000, Diao et al. 2008).

Literature References

PubMed ID	Title	Journal	Year
9753325	Cdc2 kinase directly phosphorylates the cis-Golgi matrix protein GM130 and is required for Golgi fragmentation in mitosis Lowe, M, Rabouille, C, Nakamura, N, Watson, R, Jackman, M, Jämsä, E, Rahman, D, Pappin, DJC, Warren, G	Cell	1998
10679020	The role of the tethering proteins p115 and GM130 in transport through the Golgi apparatus in vivo Seemann, J, Jokitalo, EJ, Warren, G	Mol. Biol. Cell	2000
18167358	Coordination of golgin tethering and SNARE assembly: GM130 binds syntaxin 5 in a p115-regulated manner Diao, A, Frost, L, Morohashi, Y, Lowe, M	J. Biol. Chem.	2008