PLK1 phosphorylates GORASP1

Stable Identifier
R-HSA-2214351
Type
Reaction [transition]
Species
Homo sapiens
Compartment
Synonyms
Activation of the Anaphase Promoting Complex (APC) by PLK1
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CDK1-mediated phosphorylation of GORASP1 (GRASP65) enables GORASP1 to recruit PLK1 (Preisinger et al. 2005). PLK1 phosphorylates GORASP1 on serine residue S189 (Sengupta and Linstedt 2010). This serine residue is near the GORASP1 region involved in GORASP1 dimerization and oligomerization, a process underlying the stacking of cis-Golgi cisternae (Wang et al. 2003). The phosphorylation of S189 by PLK1 impairs Golgi cisternae stacking (tethering), contributing to Golgi unlinking and fragmentation in mitosis, probably by preventing formation of GORASP1 dimers and oligomers (Sutterlin et al. 2001, Sengupta and Linstedt, 2010). Two other potential phosphorylation sites that match PLK1 substrate consensus sequence exist in GORASP1, but their functional significance has not yet been examined (Sengupta and Linstedt, 2010).

Literature References
PubMed ID Title Journal Year
20937827 Mitotic inhibition of GRASP65 organelle tethering involves Polo-like kinase 1 (PLK1) phosphorylation proximate to an internal PDZ ligand

Sengupta, D, Linstedt, AD

J. Biol. Chem. 2010
11447294 Polo-like kinase is required for the fragmentation of pericentriolar Golgi stacks during mitosis

Sutterlin, C, Lin, CY, Feng, Y, Ferris, DK, Erikson, RL, Malhotra, V

Proc Natl Acad Sci U S A 2001
15678101 Plk1 docking to GRASP65 phosphorylated by Cdk1 suggests a mechanism for Golgi checkpoint signalling

Preisinger, C, Korner, R, Wind, M, Lehmann, WD, Kopajtich, R, Barr, FA

EMBO J 2005
12839990 A direct role for GRASP65 as a mitotically regulated Golgi stacking factor

Wang, Y, Seemann, J, Pypaert, M, Shorter, J, Warren, G

EMBO J. 2003
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Catalyst Activity
Title
protein serine/threonine kinase activity of p-T216,S274,S373-GORASP1:p-S37-GOLGA2:p-RAB1:GTP:p-T210-PLK1 [Golgi membrane]
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