Reactome | Transport of antigen loaded MHC II molecules to surface

Transport of antigen loaded MHC II molecules to surface

Stable Identifier

R-HSA-2213248

Type

Reaction [omitted]

Species

Homo sapiens

Compartment

lysosomal membrane, plasma membrane, cytosol

ReviewStatus

5/5

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Transport of antigen loaded MHC II molecules to surface

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Once MHC class II-peptide complexes are formed, they must be transported back to the cell surface. It is unclear how this occurs. LEs/lysosomes with peptide loaded MHC II molecules may move in a bidirectional manner in a stop-and-go fashion along microtubules to the plasma membrane, driven by the activities of the oppositely-directed motor proteins dynein and kinesin (Wubbolts et al. 1996, 1999, Chow et al. 2002, Rocha & Neefjes 2007). Ultimately, LEs/lysosomes fuse to the plasma membrane delivering the MHC II-peptide complexes to the surface (Raposo et al. 1996, Rocha & Neefjes 2007). RAB7-GTP present on LEs/lysosome membrane interacts with Rab7-interacting lysosomal protein (RILP) and oxysterol-binding protein-related protein 1L (ORP1L) to form a tripartite RILP-Rab7-ORP1L complex. RILP binds to the p150 dynactin subunit to recruit the dynein or kinesin motor proteins. ORP1L recruits this complex to betaIII spectrin domains, which appears to be critical for dynein motor activation and transport of LEs/lysosome vesicles to the cell periphery (Johansson et al. 2007, Rocha & Neefjes 2008).

Literature References

PubMed ID	Title	Journal	Year
18046453	MHC class II molecules on the move for successful antigen presentation Neefjes, J, Rocha, N	EMBO J	2008
10036229	Opposing motor activities of dynein and kinesin determine retention and transport of MHC class II-containing compartments Neefjes, J, Reits, E, Vallee, RB, Fernandez-Borja, M, Dusseljee, S, Jordens, I, Wubbolts, R, Echeverri, C	J Cell Sci	1999
8642258	B lymphocytes secrete antigen-presenting vesicles Harding, CV, Liejendekker, R, Stoorvogel, W, Nijman, HW, Geuze, HJ, Melief, CJ, Raposo, G	J Exp Med	1996
19564404	Cholesterol sensor ORP1L contacts the ER protein VAP to control Rab7-RILP-p150 Glued and late endosome positioning Janssen, H, Janssen, L, Rocha, N, Zwart, W, Neefjes, J, Kuijl, C, Houben, D, van der Kant, R	J. Cell Biol.	2009
8909537	Direct vesicular transport of MHC class II molecules from lysosomal structures to the cell surface Neefjes, J, Tulp, A, Calafat, J, Fernandez-Borja, M, Janssen, H, Verwoerd, D, Dusseljee, S, Wubbolts, R, Oomen, L	J Cell Biol	1996
17283181	Activation of endosomal dynein motors by stepwise assembly of Rab7-RILP-p150Glued, ORP1L, and the receptor betalll spectrin Olkkonen, VM, Kuijl, C, Johansson, M, Neefjes, J, Zwart, W, Janssen, L, Jordens, I, Rocha, N	J Cell Biol	2007
12198549	Dendritic cell maturation triggers retrograde MHC class II transport from lysosomes to the plasma membrane Toomre, D, Mellman, I, Chow, A, Garrett, W	Nature	2002