Arachidonic acid is oxidised to 15S-HpETE by ALOX15/15B

Stable Identifier
R-HSA-2162002
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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Arachidonate 15-lipoxygenase (ALOX15) (Gulliksson et al. 2007, Kuhn et al. 1993, Izumi et al. 1991) and arachidonate 15-lipoxygenase B (ALOX15B) (Tang et al. 2002, Wecksler et al. 2008) are lipid peroxidising enzymes mainly expressed in airway epithelial cells, eosinophils, reticulocytes and in macrophages. They insert molecular oxygen at C-6 from the omega-end of arachidonic acid with formation of the unstable intermediate 15S-hydroperoxyeicosatetraenoic acid (15S-HpETE) which can be further converted, enzymatically or non-enzymatically, to 15S-hydroxyeicosatetraenoic acid (15S-HETE).

Literature References
PubMed ID Title Journal Year
1662607 Purification of two forms of arachidonate 15-lipoxygenase from human leukocytes

Izumi, T, Rådmark, O, Jörnvall, H, Samuelsson, B

Eur J Biochem 1991
18570379 Substrate specificity changes for human reticulocyte and epithelial 15-lipoxygenases reveal allosteric product regulation

Wecksler, AT, Kenyon, V, Deschamps, JD, Holman, TR

Biochemistry 2008
8334154 Overexpression, purification and characterization of human recombinant 15-lipoxygenase

Kühn, H, Barnett, J, Grunberger, D, Baecker, P, Chow, J, Nguyen, B, Bursztyn-Pettegrew, H, Chan, H, Sigal, E

Biochim Biophys Acta 1993
11839751 Evidence that arachidonate 15-lipoxygenase 2 is a negative cell cycle regulator in normal prostate epithelial cells

Tang, S, Bhatia, B, Maldonado, CJ, Yang, P, Newman, RA, Liu, J, Chandra, D, Traag, J, Klein, RD, Fischer, SM, Chopra, D, Shen, J, Zhau, HE, Chung, LW, Tang, DG

J Biol Chem 2002
17662651 Expression of 15-lipoxygenase type-1 in human mast cells

Gulliksson, M, Brunnström, A, Johannesson, M, Backman, L, Nilsson, G, Harvima, I, Dahlén, B, Kumlin, M, Claesson, HE

Biochim Biophys Acta 2007
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
arachidonate 15-lipoxygenase activity of ALOX15/15B [cytosol]
Physical Entity
Activity
Orthologous Events
Cross References
Rhea
Authored
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Created