Duplex siRNA is loaded into Argonaute

Stable Identifier
Reaction [omitted]
Homo sapiens
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Following cleavage the duplex siRNA reoriented on DICER1 and then transferred from DICER1 to an Argonaute protein (AGO1, AGO2, AGO3, or AGO4) within the RISC loading complex (RLC).

Literature References
PubMed ID Title Journal Year
20972213 A role for human Dicer in pre-RISC loading of siRNAs

Sakurai, K, Song, MS, Alluin, J, Rossi, JJ, Heale, B, Kim, DH, Gatignol, A, Behlke, MA, Amarzguioui, M

Nucleic Acids Res. 2011
16357216 A human, ATP-independent, RISC assembly machine fueled by pre-miRNA

Mourelatos, Z, Maniataki, E

Genes Dev 2005
22106413 Dicer is dispensable for asymmetric RISC loading in mammals

Betancur, JG, Tomari, Y

RNA 2012
25793518 DICER-ARGONAUTE2 complex in continuous fluorogenic assays of RNA interference enzymes

Wang, L, Bernard, MA, Tachado, SD

PLoS ONE 2015
15973356 TRBP recruits the Dicer complex to Ago2 for microRNA processing and gene silencing

Gregory, RI, Shiekhattar, R, Chendrimada, TP, Norman, J, Nishikura, K, Cooch, N, Kumaraswamy, E

Nature 2005
23661684 Differential roles of human Dicer-binding proteins TRBP and PACT in small RNA processing

Zhou, K, Doudna, JA, Noland, CL, Smith, AM, Lee, HY

Nucleic Acids Res. 2013
23511973 The rough endoplasmatic reticulum is a central nucleation site of siRNA-mediated RNA silencing

Hean, J, Aeschimann, F, Basselet, P, Stalder, L, Hintersteiner, M, Heusermann, W, Meisner-Kober, NC, Wirz, J, Morrissey, DV, Sokol, L, Pfanzagl, V, Trojer, D, Fritzsche, A, Weiler, J

EMBO J. 2013
16142218 TRBP, a regulator of cellular PKR and HIV-1 virus expression, interacts with Dicer and functions in RNA silencing

Filipowicz, W, Gatignol, A, Zhang, H, Laine, S, Haase, AD, Jaskiewicz, L, Sack, R

EMBO Rep 2005
18842624 Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy reveal the cytoplasmic origination of loaded nuclear RISC in vivo in human cells

Schwille, P, Mütze, J, Crell, K, Weinmann, L, Staroske, W, Höck, J, Ohrt, T, Meister, G

Nucleic Acids Res 2008
17531811 RNA helicase A interacts with RISC in human cells and functions in RISC loading

Rana, TM, Robb, GB

Mol Cell 2007
19966796 ATP-dependent human RISC assembly pathways

Kawamata, T, Iwasaki, S, Ye, X, Paroo, Z, Tomari, Y, Yoda, M, Liu, Q

Nat Struct Mol Biol 2010
21726814 siRNA repositioning for guide strand selection by human Dicer complexes

Ma, E, Doudna, JA, Noland, CL

Mol. Cell 2011
18178619 In vitro reconstitution of the human RISC-loading complex

Ma, E, Zhou, M, Robinson, CV, Doudna, JA, MacRae, IJ

Proc Natl Acad Sci U S A 2008
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