Duplex miRNA is loaded into Argonaute

Stable Identifier
R-HSA-2106614
Type
Reaction [omitted]
Species
Homo sapiens
Compartment
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The duplex miRNA (designated miRNA-miRNA*) is reoriented on DICER1 after cleavage and then transferred from DICER1 to an Argonaute protein (AGO2 or, by inference, AGO1, AGO3, or AGO4) within the RISC loading complex. Particular Argonaute proteins do not appear to have significantly different populations of miRNAs, however Argonaute identity can affect the resulting length of the miRNA.

Literature References
PubMed ID Title Journal Year
16142218 TRBP, a regulator of cellular PKR and HIV-1 virus expression, interacts with Dicer and functions in RNA silencing

Haase, AD, Jaskiewicz, L, Zhang, H, Laine, S, Sack, R, Gatignol, A, Filipowicz, W

EMBO Rep 2005
23511973 The rough endoplasmatic reticulum is a central nucleation site of siRNA-mediated RNA silencing

Stalder, L, Heusermann, W, Sokol, L, Trojer, D, Wirz, J, Hean, J, Fritzsche, A, Aeschimann, F, Pfanzagl, V, Basselet, P, Weiler, J, Hintersteiner, M, Morrissey, DV, Meisner-Kober, NC

EMBO J. 2013
16357216 A human, ATP-independent, RISC assembly machine fueled by pre-miRNA

Maniataki, E, Mourelatos, Z

Genes Dev 2005
22106413 Dicer is dispensable for asymmetric RISC loading in mammals

Betancur, JG, Tomari, Y

RNA 2012
22844086 microRNAs associated with the different human Argonaute proteins

Dueck, A, Ziegler, C, Eichner, A, Berezikov, E, Meister, G

Nucleic Acids Res. 2012
25793518 DICER-ARGONAUTE2 complex in continuous fluorogenic assays of RNA interference enzymes

Bernard, MA, Wang, L, Tachado, SD

PLoS ONE 2015
19966796 ATP-dependent human RISC assembly pathways

Yoda, M, Kawamata, T, Paroo, Z, Ye, X, Iwasaki, S, Liu, Q, Tomari, Y

Nat Struct Mol Biol 2010
15973356 TRBP recruits the Dicer complex to Ago2 for microRNA processing and gene silencing

Chendrimada, TP, Gregory, RI, Kumaraswamy, E, Norman, J, Cooch, N, Nishikura, K, Shiekhattar, R

Nature 2005
21726814 siRNA repositioning for guide strand selection by human Dicer complexes

Noland, CL, Ma, E, Doudna, JA

Mol. Cell 2011
23661684 Differential roles of human Dicer-binding proteins TRBP and PACT in small RNA processing

Lee, HY, Zhou, K, Smith, AM, Noland, CL, Doudna, JA

Nucleic Acids Res. 2013
18178619 In vitro reconstitution of the human RISC-loading complex

MacRae, IJ, Ma, E, Zhou, M, Robinson, CV, Doudna, JA

Proc Natl Acad Sci U S A 2008
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