SLC1A1-3,6,7 exchange L-Glu, H+ and 3Na+ for K+

Stable Identifier
Reaction [transition]
Homo sapiens
L-Glutamate uptake by neurons
Locations in the PathwayBrowser
SVG |   | PPTX  | SBGN
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout
There are two classes of glutamate transporters; the excitatory amino acid transporters (EAATs) which depend on an electrochemical gradient of Na+ ions and vesicular glutamate transporters (VGLUTs) which don't. Together, these transporters uptake and release glutamate to mediate this neurotransmitter's excitatory signal and are part of the glutamate-gluatamine cycle. The SLC1 gene family includes five high-affinity glutamate transporters encoded by SLC1, 2, 3, 6 and 7. These transporters can mediate transport of L-Glutamate (L-Glu), L-Aspartate and D-Aspartate with cotransport of 3 Na+ ions and H+ and antiport of a K+ ion. This mechanism allows glutamate into cells against a concentration gradient thus excess L-Glu released by the pre-synaptic neuron in the synaptic cleft is cleared. This is a crucial factor in the protection of neurons against glutamate excitotoxicity in the CNS. SLC1A2 and 3 are mainly expressed by astrocytes whereas SLC1A1 and 6 are predominantly neuronal.
SLC1A1 is expressed throughout the CNS however SLC1A6 is predominantly localized to purkinje cells. SLC1A7 is highly expressed in rod photoreceptor and bipolar cells of the retina. Astrocytic SLC1As are expressed in astrocytes in close apposition to the synapses and neuronal SLC1As are expressed in the extra-synaptic or peri-synaptic locations on the neurons. Astrocytic SLC1As are responsible for majority of the glutamate uptake, neuronal transporters are responsible for glutamate clearance in specialized synapses in cerebellum where the spatial relationship between the glutamate receptors and SLC1As is altered and glutamate receptors are expressed in the peri-synaptic region (Zhou & Danbolt 2014).
Defects in the SLC1A1 gene may be a cause of dicarboxylicamino aciduria (glutamate-aspartate transport defect in the kidney and intestine) (Jen et al. 2005).
PRA1 family protein 3 (ARL6IP5 aka ADP-ribosylation factor-like protein 6-interacting protein 5) is a microtuble-associated protein that is able to regulate intracellular concentrations of glutamate as well as tuarine. It negatively regulates SLC1A1 by decreasing its affinity for glutamate (L-Glu). The activity of human SLC1A1 is based on similarity to rat Eaac1 (aka GTRAP3-18) (Lin et al. 2001).
Literature References
PubMed ID Title Journal Year
24578174 Glutamate as a neurotransmitter in the healthy brain

Zhou, Y, Danbolt, NC

J Neural Transm 2014
11242046 Modulation of the neuronal glutamate transporter EAAC1 by the interacting protein GTRAP3-18

Rothstein, JD, Lin, CI, Dykes-Hoberg, M, Lee, A, Ruggiero, AM, Jackson, M, Orlov, I

Nature 2001
16116111 Mutation in the glutamate transporter EAAT1 causes episodic ataxia, hemiplegia, and seizures

Wan, J, Howard, BD, Jen, JC, Baloh, RW, Palos, TP

Neurology 2005
Catalyst Activity

high-affinity glutamate transmembrane transporter activity of SLC1A1-3,6,7 [plasma membrane]

This event is regulated
Negatively by
Orthologous Events
Cross References
Cite Us!