Reactome | DHPS tetramer synthesizes Dhp-K50-EIF5A from EIF5A and spermidine

DHPS tetramer synthesizes Dhp-K50-EIF5A from EIF5A and spermidine

Stable Identifier

R-HSA-204647

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

Synonyms

EIF5A + spermidine <=> EIF5A(Dhp) + 1,3-diaminopropane

ReviewStatus

5/5

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DHPS tetramer synthesizes Dhp-K50-EIF5A from EIF5A and spermidine

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Cytosolic deoxyhypusine synthase (DHPS) tetramer catalyzes the reaction of of EIF5A protein, spermidine (SPM), and NAD+ to convert lysine 50 of EIF5A to deoxyhypusine (Dhp), generating 1,3 diaminopropane, NADH and H+ in the process (Clement et al. 2003; Joe et al. 1995; Park 2006; Wolff et al. 1997). Although the reaction is reversible, the reverse reaction is probably minimized under physiological conditions by the rapid, irreversible conversion of EIF5A Dhp residues to hypusine.

Literature References

PubMed ID	Title	Journal	Year
9188485	Enzyme-substrate intermediate formation at lysine 329 of human deoxyhypusine synthase Folk, JE, Wolff, EC, Park, MH	J Biol Chem	1997
7673224	Cloning and expression of human deoxyhypusine synthase cDNA. Structure-function studies with the recombinant enzyme and mutant proteins. Wolff, EC, Park, MH, Joe, YA	J Biol Chem	1995
14622290	Identification and characterization of eukaryotic initiation factor 5A-2 Wolff, EC, Henderson, CA, Park, MH, Johansson, HE, Hershey, JW, Clement, PM, Smit-McBride, Z, Jenkins, ZA	Eur J Biochem	2003
16452303	The post-translational synthesis of a polyamine-derived amino acid, hypusine, in the eukaryotic translation initiation factor 5A (eIF5A) Park, MH	J Biochem (Tokyo)	2006