DHPS tetramer synthesizes Dhp-K50-EIF5A from EIF5A and spermidine

Stable Identifier
Reaction [transition]
Homo sapiens
EIF5A + spermidine <=> EIF5A(Dhp) + 1,3-diaminopropane
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Cytosolic deoxyhypusine synthase (DHPS) tetramer catalyzes the reaction of of EIF5A protein, spermidine (SPM), and NAD+ to convert lysine 50 of EIF5A to deoxyhypusine (Dhp), generating 1,3 diaminopropane, NADH and H+ in the process (Clement et al. 2003; Joe et al. 1995; Park 2006; Wolff et al. 1997). Although the reaction is reversible, the reverse reaction is probably minimized under physiological conditions by the rapid, irreversible conversion of EIF5A Dhp residues to hypusine.

Literature References
PubMed ID Title Journal Year
9188485 Enzyme-substrate intermediate formation at lysine 329 of human deoxyhypusine synthase

Folk, JE, Wolff, EC, Park, MH

J Biol Chem 1997
7673224 Cloning and expression of human deoxyhypusine synthase cDNA. Structure-function studies with the recombinant enzyme and mutant proteins.

Wolff, EC, Park, MH, Joe, YA

J Biol Chem 1995
14622290 Identification and characterization of eukaryotic initiation factor 5A-2

Wolff, EC, Henderson, CA, Park, MH, Johansson, HE, Hershey, JW, Clement, PM, Smit-McBride, Z, Jenkins, ZA

Eur J Biochem 2003
16452303 The post-translational synthesis of a polyamine-derived amino acid, hypusine, in the eukaryotic translation initiation factor 5A (eIF5A)

Park, MH

J Biochem (Tokyo) 2006
Catalyst Activity

deoxyhypusine synthase activity of DHPS tetramer [cytosol]

Orthologous Events
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