DHPS tetramer synthesizes Dhp-K50-EIF5A from EIF5A and spermidine

Stable Identifier
R-HSA-204647
Type
Reaction [transition]
Species
Homo sapiens
Compartment
Synonyms
EIF5A + spermidine <=> EIF5A(Dhp) + 1,3-diaminopropane
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Cytosolic deoxyhypusine synthase (DHPS) tetramer catalyzes the reaction of of EIF5A protein, spermidine (SPM), and NAD+ to convert lysine 50 of EIF5A to deoxyhypusine (Dhp), generating 1,3 diaminopropane, NADH and H+ in the process (Clement et al. 2003; Joe et al. 1995; Park 2006; Wolff et al. 1997). Although the reaction is reversible, the reverse reaction is probably minimized under physiological conditions by the rapid, irreversible conversion of EIF5A Dhp residues to hypusine.

Literature References
PubMed ID Title Journal Year
7673224 Cloning and expression of human deoxyhypusine synthase cDNA. Structure-function studies with the recombinant enzyme and mutant proteins.

Joe, YA, Wolff, EC, Park, MH

J Biol Chem 1995
9188485 Enzyme-substrate intermediate formation at lysine 329 of human deoxyhypusine synthase

Wolff, EC, Folk, JE, Park, MH

J Biol Chem 1997
14622290 Identification and characterization of eukaryotic initiation factor 5A-2

Clement, PM, Henderson, CA, Jenkins, ZA, Smit-McBride, Z, Wolff, EC, Hershey, JW, Park, MH, Johansson, HE

Eur J Biochem 2003
16452303 The post-translational synthesis of a polyamine-derived amino acid, hypusine, in the eukaryotic translation initiation factor 5A (eIF5A)

Park, MH

J Biochem (Tokyo) 2006
Participants
Participant Of
Event Information
Catalyst Activity
Catalyst Activity
Title
deoxyhypusine synthase activity of DHPS tetramer [cytosol]
Physical Entity
Activity
Orthologous Events
Authored
Reviewed
Created
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